Rene,
No not the quality of the slides at all. Actually my lab does not do the
processing and initial HE, which is done in our main histology lab. My
concern is that the push for quicker TATs sometimes leads to inadequate
fixation, etc., which affects our lab.I'm just saying that fast
Hello Histo World,
please share your experience from going from clinical histology to
research histology...What are the major difference? Are there complications or
pleasant surprises?
Cassandra Davis
cda...@che-east.org
302-575-8095
Confidentiality Notice:
This e-mail, including
The first BIG difference for me was the workflow. Completely different time
frames for completion of work. The next pleasant change was the time avaialble
to work with the specimens. This was many, many years ago. I enjoyed the
research work, but did not enjoy the low pay and the worry for
Hello to everyone!
Has anyone used PD-1 on FFPE tissue? Could I please get a copy of your
IHC protocol?
I would like to know:
* antigen retrieval: method, retrieval buffer and times
* primary antibody incubation: antibody diluyent (did you use detergent?
tween or other?) and incubation time.
Victoria
Cartilage can be tricky, proper processing and sectioning is important
initially. The samples can sometimes be cartilage only or both cartilage and
the underlying bone, we have worked with both. It also depends upon what
species you are working with. We have worked with mouse up to
Hi Cassie, For me, it has been mostly positive changes. I went from feeling
like a robot cranking out slides and rarely hearing about any impact on the
patient to being involved from start to finish on published articles in
research journals. I've been able to use cutting edge technology to
Having a problem with fungus on top of both the pas and gms stain. There are no
solutions used on both test. Other than distilled water we can not figure it
out. All solutions have been changed and clean containers used.
Judith Gale Pardue
Histology Supervisor
judith_pardue@memorial .org
Judith
Its been years ago but I wrote a ASCP tech sample on this - I can't remember
what solution it was but one of the solutions we used for the GMS stain
actually grew fungus in it and we were getting staining on top of the tissue
sections also. If you don't think it's one of your solutions
Hi Judith, If you have made completely fresh solutions, even any stock reagents
and still have the problem, then the fungus may be arriving from another
source. I once had a problem of pollen landing on my water bath and showing up
on the stains that would detect it. You may have fungus on
Hello Histonet-
I was just curious as to what types of Deionized water systems you all use.
We are looking for an easy, low-volume system that creates Type II DI
water. Any information would be greatly appreciated. Thank you!
Regards,
Erin Sarricks, HT(ASCP)
Cassie,
It is so much better!
Basically you are doing the same thing but in my instance I get a pretty wide
variety of projects (different species) and pretty often need to address each
one differently.
I have many different processing schedules programmed into my processor - like
whole
Can anyone tell me what paraffin is best substitute for Tissue Prep 2? They
tell me it is not being manufactured anymore. And will we have to revalidate
all IHCs?
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Two things I have seen dealt with 1) distilled water filter had not been
changed and was growing fungus 2) tech cutting slides had not changed water in
waterbath for I while.
Cassandra Davis
cda...@che-east.org
302-575-8095
Saint Francis Hospital
Saintfrancishealthcare.org
Saint Francis
Hello to all in histoland. Does any one have a procedure for storage and
labeling of formalin? Any help would be appreciated
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-2148
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received
Judith,
What counterstain are you using for each? If it is the light green, then that
may be the culprit. Also check your baskets that you to deparaffinze, they may
need to be cleaned.
Sincerely,
Toysha N. Mayer, MBA, HT(ASCP)
tnma...@mdanderson.org
Instructor/Education Coordinator
Program
I have had fungal contamination in the Fast Green counterstain from Thermo.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this transmission may contain privileged and
The solution that grows fungus is the light green counterstain.
Are you using that for your PAS and the GMS? When we did PAS for fungus, we
used light green instead of hematoxylin to counterstain.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
Busy Dermatopathology laboratory located in West Palm Beach looking
for full time Histotech. No Mohs. Contact Dr. Morales with questions.
561-653-8005.
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