Cheryl
Yes, I understand. It is highly likely this will be the way things go here
also.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: cmil...@physlab.com
To: joellewea...@hotmail.com
CC: histonet-boun...@lists.utsouthwestern.edu;
histonet@lists.utsouthwestern.edu
Date: Wed, 12 Feb 2014
Hi Everyone!
I was wondering if any of you have a favorite CD68 macrophage antibody that you
have used on mouse tissue and is especially reliable. This is a target that
has been notorious in our lab and I was wondering if there was a really good
one to use.
Thanks in advance for your help!
We discontinued them over a year ago.
Martha Ward, MT (ASCP) QIHC
Manager
Molecular Diagnostics Lab
Medical Center Boulevard \ Winston-Salem, NC 27157
p 336.716.2109 \ f 336.716.5890
mw...@wakehealth.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi,
How are you guys doing? Please I am wondering if some of you guys are using
this type of Leica cryostat and would be interested in the decontamination
procedure that you have in place.
Thanks,
Adesupo Adesuyi, BSMT, HT (ASCP), HTL (ASCP), QIHC (ASCP)
Histology Supervisor
Norman
Hi Anna,
Your subject line is F4/80 and you are asking for CD68. I thought they are
different antibodies, but I may be wrong.
We have never been able to find CD68 ab that works well of FFPE mouse
tissues. Recently we had success with F4/80 Rat anti-mouse Clone: BM8 from
BioLegend. It only worked
We also stopped running negative controls more than a year ago.
Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
Dubuque, IA
From: histonet-boun...@lists.utsouthwestern.edu
Wow! Three things!
Negative IHC Controls - Since we use a polymer detection system, we long
ago quit using negative controls as a cost savings measure. I can't
imagine doing anything less considering the cost. It has saved us
thousands.
UV cryostat decontamination - Unfortunately, if you are
So, Ventana came out to my lab to do a LEAN consult for us since we are
building a new lab...they want to sell us their vantage tracking system and HE
stainer. Our Rep is coming on Fri to talk to me, our lab director and CEO
about these products. Anyone using these? I'd like some feedback
We had success with CD68 on mouse tissue from Boster cat# PA1518. For F4/80 we
use the rat anti-mouse antibody from eBioscience cat# 14-4801.
Bea
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-Original
I agree with most of what others have said concerning your question Anna, but I
have another BIG issue that no one has mentioned:
Heating Bouin's in the Microwave. This practice is very unsafe! Bouin's
(picric acid) is highly explosive and should NEVER be microwaved!
Granted this is my opinion,
We are only 2 weeks from the ASH Annual Spring Meeting in Hot Springs AR. We
are excited and ready to welcome everyone to our 2 day meeting for Histology
CEUs and Roaring Twenties Meet and Greet.
Registration will open through both days on-site and anyone wishing to
pre-register
Rat anti ms F4/80 is used on mouse tissue ffpe for macrophages not anti
human cd68.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
-Original Message-
From:
Hello All,
I have been using a procollagen antibody from Millipore and the latest lots now
do not work. I am in the middle of running a clinical study which has a
deadline that is closing in and would greatly appreciate if anyone could
recommend a good procollagen antibody for skin. This
We use a Leica CM1850 currently. May differer a bit. On a daily basis, we clean
the loose trimmings and wipe the inside with 95% ethanol. The exterior surfaces
are cleaned daily with caviwipes.
Once a month we turn off the machine, remove the microtome (I don't know if
this is proper for the
For the most part I like this system. A few things though. Their HE
racks constantly break so make sure in your contract you get free ones
to replace them. Also if you have to remove a coverslip it is a pain.
You have to heat the slide for a while so see if they will include a
heater and you
Kris
We have had good success with Procollagen Type I from Abcam ab64409 on human
skin samples.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
Seems I've mis-spoken on this one. I realize that Bouin's is only explosive
when dried, but drying could occur during the process of microwaving. Also,
we use Saturated picric acid for our mordant rather than Bouin's to also not
have the exposure to the formalin fumes associated with another
Hello, I agree anti-human CD68 might not be best for mouse tissue but there are
specific mouse CD68 (the mouse homolog to human CD68) antibodies out there.
FA-11 is one of my favorites. Have used both F4/80 and (rat anti-mouse CD68)
on flow, frozens and FFPE. Anna, a literature search gives
I'll chime in here and state that we have used F4/80, iba-1, and CD68 for mouse
macrophages, for CD68 we use the same clone as Ray is talking about we get that
particular antibody from Serotec. All work on FFPE tissues in our hands.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier
http://izmirpeyzaj.gen.tr/res/twit.php?bktkkae1280eeku
sarahholt...@yahoo.com
Thu, 13 Feb 2014 02:12:43
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