[Histonet] Bone Histology Protocol

Hello colleagues! I'm currently trying to construct a complete Bone Processing Protocol that includes fixation (10%NBF), decalcification (EDTA), Dehydration, Clearing of the decalcificant (Xylene), infiltration, and Embedding in Paraffin. I would like to look at some procedures just to get a goo

[Histonet] herbert skip brown

Skip, Please reply to me... Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.mor...@ucsfmedctr.org

RE: [Histonet] Input on Sakura E150 Purchase

We have a Sakura tissue processor that is more than 25 years old and still working very well with annual preventative maintenance. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-

Re: [Histonet] question about refrigeration

Hi Peggy, In my opinion, it depends upon the objective of the study. Cell autolysis begins shortly after death as a result of lack of oxygen supply as well as nutrients. The membranes of the lysosomes break down, and the acid hydrolases begin to degrade the cellular If you're merely looking

[Histonet] Mouse NK cell marker

Good day, colleagues! Does anyone have a good Natural Killer cell marker for mouse tissue? Any antibody recommendations/protocol information would be a big help as well. This is for IHC, not IF. Thanks in advance! Ashley Troutman BS, MBA, HT(ASCP)QIHC Supervisor-Translational Pathology Share

[Histonet] Wierd hemotoxylin result on my macaque liver slide

Hi everyone. I was staining a FFPE macaque liver tissue slide with hematoxylin the other day, and it had an odd appearance. All the cells stained the usual way with the blue nuclear staining that you expect with hematoxylin. But a few randomly scattered individual cells throughout the tissue s

RE: [Histonet] Input on Sakura E150 Purchase

Another +1 for the VIP E300 (very similar model). We have one that's close to 20 years old that's still running. It has an annual PM and any repairs have been minor. I have been told that part availability isn't yet an issue with this model. Michael J. Dessoye, M.S. | Histology/Toxicology/R

[Histonet] RE: Slides for control/patient

We like Fisher's Red Control Slide with Adhesion #22-042-910 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, February 28, 2014 9:58 AM To: histonet@lists.utsouthwestern.edu Sub

[Histonet] Slides for control/patient

Happy Friday! I was wondering, for the labs that are using the slides for IHC that have a place for the control tissue and the patient tissue, which vendor would you recommend. I also would not mind being contacted directly by vendors who wish to inform me of their products. Thanks, Toni __

[Histonet] question about refrigeration

Histonetters, I was wondering how long a bone specimen (portion of pt's tibia in this case) can be in a refrigerator before it will decompose and not produce a good quality section? The case was completed at 3:30 pm, refrigerated and then put into formalin at 8:15 am the next day? I am pushin

[Histonet] RE: Neutralization of AZF

I would like to know the answer to this too. Haley Huggins Haley Huggins, HT(ASCP)cm Pathology/Histology Manager Marian Medical Center 1400 East Church St Santa Maria, CA 93454 805-739-3170 (path lab) 805-739-3153 (office) 303-652-7453 (cell) 805-332-8697 (rightfax) -Original Message-