[Histonet] RE: Cornflaking artifact

2014-03-12 Thread Sharon Scalise
I am looking for help with "cornflaking" (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make

[Histonet] TEM choices

2014-03-12 Thread Steven Weston
HI there fellow histonetters, We are in the fortunate position of being able to purchase a new TEM and was wondering what you all think of the present choices on market. We wish to get a digital machine to replace an ageing Joelle. Any feedback from users about recent purchases would be welcome,

[Histonet] RE: Re: Pathology Protocols

2014-03-12 Thread Joe W. Walker, Jr.
Donna brings up a good point. I too was able to remove a 0.5 FTE from my budget line once we went to a paperless system. This might help convince the pathologist to get on board with the paperless process. We did have a pathologist who helped user in the changes with the rest of the group. J

[Histonet] Looking for PEDV antibody vendor

2014-03-12 Thread Jan Shivers
Hello all, Can anyone suggest a stateside vendor for Porcine Epidemic Diarrhea Virus antibody? My overseas vendor is discontinuing their veterinary products and I'm in great need of a constant and reliable supply. Thanks in advance, -- Jan Shivers Senior Scientist IHC/Histology Section Head Pat

[Histonet] Paraffin type and Tetracycline labelling Questions

2014-03-12 Thread Wait, Trevor Jordan
For those who have done Decalcified bone processing with paraffinwhat is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label?

Re: [Histonet] Alizarin Red on undecalcified bone

2014-03-12 Thread Sean McBride
Hi Mesru, 1. Cryosectioning followed by Alizarin red staining may work. I'm not well-versed in this technique, so I will let other experts in this technique guide you. 2. Alizarin red functions by binding to calcium ions. Acids or chelating agents used in de-mineralization processes may ve

[Histonet] retraction of mounting medium

2014-03-12 Thread path lab
Has anyone experienced retraction of mounting medium from the edges of the slides (not yet reaching tissue but the retraction could conceivably continue until tissue is compromised) after long term (greater than 3 years) storage using recommended mounting media for automatic coverslippers? How hav

[Histonet] CAP Annual Results Comparison for FISH/ISH

2014-03-12 Thread O'neil, Beth
Would fellow Histonetters be able to explain how they answer the following CAP question: ANP.22970 For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates in

[Histonet] HISTOPALOOZA APRIL 25 - APRIL 27 GEORGIA SOCIETY FOR HISTOTECHNOLOGY

2014-03-12 Thread Zimmerman, Billie
The Histopalooza is fast approaching and you need to secure your room at the Lodge and Spa at Callaway Gardens, Ga. If rooms are full, I suggest the cottages which are within steps of the Lodge. The contact person for securing your room is Taylor Reames 706-489-3359. I'm looking forward to some

[Histonet] Alizarin Red on undecalcified bone

2014-03-12 Thread Mesru T
Dear Histology Experts, I need your help. I am trying to stain undecalcified bone (adult mouse limb) with Alizarin Red S. I was thinking about two options: 1. Fixed frozen sections with Cryojane tape transfer system then staining the sections with Alizarin Red. 2. Alcohol fixed whole limb (whole

[Histonet] RE: Xylene-free (isopropanol) processing protocol

2014-03-12 Thread Willis, Donna G.
What type of processing instrument are you using? Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.wil...@baylorhealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.e

[Histonet] Xylene-free (isopropanol) processing protocol

2014-03-12 Thread Justin.Peters
Is there anyone willing to share their processing protocol for using isopropanol without xylene? Justin Peters, HTL, QIHC (ASCP)CM Histology Supervisor Henrico Doctors' Hospital - Forest, Parham, and Retreat Campuses ___ Histonet mailing list Histonet@

AW: [Histonet] Decal and molecular testing

2014-03-12 Thread Gudrun Lang
Hi, we had success with 5% formic acid on trephine bone marrow for DNA-PCR (BRAF, c-kit mutation) and RNA-ISH (kappa), but had no success for FISH-testing. Perhaps someone can recommend a method, that also supports FISH? Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.ut

[Histonet] RE: Vented containers for microwave

2014-03-12 Thread Willis, Donna G.
This will depend on the microwave brand that is being used. Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.wil...@baylorhealth.edu -Original Message- From: histonet-boun...@lists.utsouth

RE: [Histonet] CEU's

2014-03-12 Thread Katelin Lester
Sakura does one free webinar per month. http://www.sakura-americas.com/about/webinars.lasso Also, if you have a maintenance contact with some companies, such as Milestone Medical, they may a offer webinar series. Katelin, HTL On Mar 12, 2014 3:49 AM, "Campbell, Tasha M." wrote: > Leica has free

[Histonet] Performance Rounding questions for Histology

2014-03-12 Thread Sara Baldwin/mhhcc.org
Any one out there engaging in Baldridge Excellence program? We are going to start rounding (like physicians) with our employe= es and we need specific questions I need to ask. So I thought what be tter group to ask?? Histonetters!! I need safety, education, obstacle=

[Histonet] Vented containers for microwave

2014-03-12 Thread Abbott, Tanya
What sort of vented containers is everyone using for microwave Special stains? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any

[Histonet] Mississippi Society for Histotechnology 2014 Meeting

2014-03-12 Thread Amber McKenzie
The Mississippi Society for Histotechnology meeting will be held in Hattiesburg, MS on Saturday, June 28th, 2014. All is welcome! If you have any questions, please feel free to contact me. Thanks! ___ Histonet mailing list Histonet@lists.utsouth

[Histonet] RE: IHC Validation

2014-03-12 Thread Barbara Tibbs
On my IHC validation documents I have a space for comments. If I change anything in a protocol I make a note in the comments space. I haven't had any issue with any accrediting agencies. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accurated

RE: [Histonet] CEU's

2014-03-12 Thread Campbell, Tasha M.
Leica has free webinars monthly. And Medpage Today has some free CEU's and also try FreeCME Weekly. I just google free CEU's and hope to find stuff. You can use the MLT stuff also, because it is a related field. You only need 2 points in the area which you are certified. And 1 point in lab or p

Re: [Histonet] Florida Society for Histotechnology 2014 Meeting

2014-03-12 Thread McDowell, Debbie
Debbie McDowell, MSA, EMT-P Business Development Manager General Data Healthcare 484.885.3901 > On Mar 11, 2014, at 8:07 PM, "JERRY SANTIAGO" wrote: > > The Florida Society for Histotechnology announces their 2014 meeting > "Histotechnology, Let the Magic Begin! at the Buena Vista Palace Hote