[Histonet] RE: Xylene testing ANP 08216

2014-04-17 Thread Michael LaFriniere
All of my labs are tested at least yearly or when we change a major procedure dealing with formalin or xylene. In addition if an associate feels the fumes are high we test that day. When I CAP inspect I like to see the lab tests annually, however, it's not the requirement but would recommend as

RE: [Histonet] IHC imaging systems

2014-04-17 Thread Michael LaFriniere
I am setting up the Ventana system and performing validation, it's taking a little longer to set up then expected but the support has been excellent. Hopefully will have it running and validated in the next few months. Validation is the time consumer I found with setting up a system. Michael R.

RE: [Histonet] IHC imaging systems

2014-04-17 Thread joelle weaver
Amen... the time investment for validation! Celebrate when it is completed :) Joelle Weaver MAOM, HTL (ASCP) QIHC > From: michael.lafrini...@ccplab.com > To: pat...@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 17 Apr 2014 12:57:37 + > Subject: RE: [Histonet] IHC imaging sy

[Histonet] RE: Waste Flammable Info Please

2014-04-17 Thread Michael LaFriniere
I found each State that I have been in has a little different method to hazardous waste handling/reporting in the laboratory. I have found that recycling alcohol, xylene and formalin greatly reduces cost; my labs demonstrate 60-70% cost reduction in all areas of concern, (usage, disposal and re

[Histonet] human cells in rat tissue

2014-04-17 Thread Marti Gaudes, Merce
Hi, good afternoon everyone, I was wondering if anybody could help me out. We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue. I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but witho

[Histonet] RE: human cells in rat tissue

2014-04-17 Thread Elizabeth Chlipala
Merce There is another Human Nuclear antibody from cell signaling we find that it works in FFPE tissues, however in our hands it did not stain all nuclei probably around 90- 95% overall. We found it to be potentially fixation dependent, we noticed that tissues that had spent longer time in fix

[Histonet] Billing Question

2014-04-17 Thread Adesupo, Adesuyi (Banjo)
Hi, Please I have a question on the new IHC Billing Policies for CPT, Medicare. My question is how do we go about the billing of the HER-2 DUAL ISH and the Kappa and Lambda ISH? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health

[Histonet] RE: Billing Question

2014-04-17 Thread Donna Millard
ISH is a different bill-code--88365. The changes were made to 88342. Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachm

RE: [Histonet] RE: human cells in rat tissue

2014-04-17 Thread Mass Histology
We use an anti-human mitochondria antibody that works well. Jim ___ James E. Staruk HT(ASCP)  www.masshistology.com    www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behal

RE: [Histonet] RE: human cells in rat tissue

2014-04-17 Thread Elizabeth Chlipala
We have used that too and it will work well in most instances, but there are some issues with that method, detection is entirely dependent upon the number of mitochondria in the cells and some cells contain more than others, as long you understand that you are fine. We feel we get a better stai

[Histonet] Xylene substitute for processing GI biopsies

2014-04-17 Thread Lori Gemeinhardt
Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining. The good, the bad

[Histonet] Re: Histonet Digest, Vol 125, Issue 20

2014-04-17 Thread DAVID OSIAGWU
Hi Gayle, i saw your response to Merissa, quite good. i have also been having problems with lectin IHC and will appreciate it if you can also forward me your Lectin IHC protocol, and your recipe for the Lectin buffer. Thank you Regards. Daniel Daniel Osiagwu, MS Dept. of Anatomic and Molecular Pa