Hi All,
We are going to optimize indoleamine 2-3 dioxygenase (IDO1) expression in
breast cancer tissue by immunohistochemistry on formalin fixed paraffin
sections.
I shall be thankful if any one please let me know about the positive control.
Regards
Muhammad Tahseen
Sr.Supervisor Histopathology
Hi Sarah,
Joyce is correct, under medicare changes in pathology coding, you can not bill
for each of the blocks of the same antibody applications, only one charge per
specific antibody mentioned in the pathology report is allowable. Medicare
immuno billing is per specimen only for each
Does anyone have any good ideas for keeping the little vials of concentrated
antibodies neat and organized in the fridge? Right now we keep ours in empty
pipette tip boxes (without tip holders) but they're always falling over and
mixed up. We use several different vendors for our concentrated
I use cardboard cryovial boxes and they work quite well. You can remove some of
the dividers to make space for the larger vials.
http://labscientific.com/Cryogenic-Supplies/Cryovial-Boxes-and-Freezer-Racks/Cryovial-Boxes-Cardboard/
Valerie Ratliff B.Sc HTL(ASCP)
Research Assistant
Hi all,
I've been having problems with cryosectioning fatty tissue for a long time now.
The section leaves a hole in the middle. I know this is probably a much
discussed topic, but I've tried a lot of strategies with no luck. The tissue is
of mouse origin and has micro-lesions buried inside
Thanks everyone for your input on processors, I think we will stay with the
VIP, it has been a good machine just getting older now.
everyone have a great day
Anita Dudley
Providence Hosp
Mobile, Alabama 36695
We got some plexiglass dividers at the Container Store if you have one
near or check online. Can't remember what they were called but they had
different sized square compartments that fit various venders antibodies.
Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Thanks Sandra! My next step was to try that.
Dakshna
- Original Message -
From: Sandra E. Esparza sespa...@seton.org
To: Dakshnapriya Balasubbramanian dakshnapr...@neo.tamu.edu
Sent: Tuesday, May 20, 2014 10:34:25 AM
Subject: RE: [Histonet] HELP- Cryosectioning FAT!
You might want to
Hi Laurie,
No, the tissue hasn't been fixed before freezing. My prof doesn't prefer the
method, but if nothing else works, we might give it a try.
Any suggested protocols for fixation?
Thanks!
Dakshna
- Original Message -
From: Laurie J King king.lau...@marshfieldclinic.org
To:
Posting for my colleague, Jeff Gordon, because his post did not go through
successfully:
Suzie, your histonet post was forwarded to me by a colleague, and I thought I
could help with your mast cell staining dilemma with pan-keratin. University
of Pennsylvania published an article a while back
We found that if using the stronger proteases/enzymes for retrievals, there
tended to be more mast cell staining seen.
Sheila Herrington
Technical Lead - Histopathology and Immunohistochemistry
Kelowna General Hospital
2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
250-862-4300 ext 7587 or 7510
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