Leica 2125
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Monday, June 02, 2014 10:44 PM
To: Sylvia Shockey
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome
Leica all the
Leica is an outstanding product. Highly recommend there microtome.
Steven Mello,HT(ASCP)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Monday, June 2, 2014 10:44 PM
To: Sylvia Shockey
Cc:
I don't have the research toggle, but my understanding while at Leica training
was that this provided a good amount of flexibility and open-ness.
Joelle Weaver MAOM, HTL (ASCP) QIHC
Date: Mon, 2 Jun 2014 13:57:57 -0700
From: wesley.mi...@gmail.com
To: histonet@lists.utsouthwestern.edu
Most companies will let you test run their newest microtomes. Let them
come to you and test for a week or so than pick the one you like best.
Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sylvia
When a microtome starts to act up the best solution is to give it an
overhaul. If with the possibility of buying one, contact Leica or Sakura first.
Both manufacture very reliable and durable instruments.
René J.
On Tuesday, June 3, 2014 10:12 AM, Anne Murvosh amurv...@advancederm.net
Leica 2125
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent:
Please give me advice on what your lab is doing for this. Thanks.
--
Rhonda Ford (HT)ASCP, Histology Lab
Henry County Hospital
(765) 521-1148
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Hartford Community College in Maryland has an online course. I would also look
at Indiana University.
Terra Wineman, HTL (ASCP)CM
Research Biologist, Nutrional Physiology
636-926-7476 phone
terra.wine...@novusint.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Harford is an AA program which would not qualify you for an HTL, unless you
already have a BS in Science, which in that case you have other avenues of
eligibility for the exam.
Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen
Hi Wes,
We have 3 bond RX units and use them for animal tissue
staining and we try all kinds of reagents (ab, chromogens, counterstains, acid
washes) . As far as the HEIR and Enzyme go Leica has their own HEIR (citrate
EDTA) and an Enzyme (PTK). You can run a Dako PTK which
Denise,
Darton College, Georgia, has an online HT degree course
http://www.darton.edu/programs/AlliedHealth/certificates/Hist-Cert.php
Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA
-Original Message-
Hey Jamie,
Thanks for the feedback. That is very useful to know. I was hoping that an
open container in the reagent rack might be used for AR, but it sounds like
we are limited to the bulk containers. This might make the difference in
choosing the Discovery Ultra over the Bond RX. Thanks again.
Rhonda,
We don't have cytology (Non GYN) specimens that get processed routinely. In
our procedure we say that it is left to the discretion of the clinician to
decide if cytology is medically necessary for the patient. We also have a list
of pathology specimens that are exempt from
My understanding is that you can use any HIER you want on the Bond RX, but
they highly recommend their own reagents since they're optimized for it.
We ended up buying a Biocare Decloaking Chamber for offline retrieval as we
some of our antibodies were suboptimally retrieved on Bond RX. However,
Sylvia,
I per diem at a lab that has four Microm HM355 S microtomes (previous
generation), and they work well; easy to get used to and dependable when
serviced properly. Your HM 325 appears to have a similar mechanism that served
their needs for years.
The Thermo Finesse is also a decent
Hi everyone,
I wouldn't give too much detail information as the histology world is very
small and everyone knows everyone.
I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went
to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs
I have
I always tell my students if you can cut, you can get a job. It appears that
you school did not properly prepare you for the demands of an average histology
job.
You need to take every opportunity to work on your craft and the major focus of
histology is cutting. With 6 to 7 years of experience
Felton, I disagree! The training this tech underwent must obviously have
covered basic histology but you cannot guarantee that a trained student will
find a laboratory that will give him/her the opportunity to develop speed while
not sacrificing quality. Having the training does not warrant
If someone promised me a brand new FREE microtome and it wasn't a LEICA, I'd
tell them, no, thanks!. I am not inclined to endorse any other brand and I
cannot imagine changing my mind about that.
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Do not give up and try not to blame a process or someone else for your lack of
developed skill at microtomy, but dedicate yourself to personal improvement.
You have 6+ years invested in a career, and if your reasons for embarking on
that career remain, then bear down and find a way to improve
Hello histonet, I was a member a number of years ago, but had gotten out of
doing much histology (I was never a pro) for awhile…. but I seem to be back at
it.
I was wondering if people might have some ideas on a good “practice tissue”,
one that I could embed, section, and stain (HE) to both
A Slim Jim would work or any meat or lunch meat purchased at the grocery store.
You will need to start by fixing the samples appropriately and then process,
embed and section. If you work in a research center that does animals studies
then I would see if you could not get some animal tissue
Bill very well put and I agree with you.
Sent from my iPhone
On Jun 3, 2014, at 4:07 PM, WILLIAM DESALVO wdesalvo@outlook.com wrote:
Do not give up and try not to blame a process or someone else for your lack
of developed skill at microtomy, but dedicate yourself to personal
Alpha, it is clear to me, after 30+ years in the field, that some are born with
the ability to cut fast AND do well at it. The rest of us just have to work
harder at developing that skill. But it does take bench time to do it. A recent
cache is that it takes 10,000 hours to become an absolute
Go to a butcher and ask what fresh offal they have. That should be much
the same as autopsy tissue. In addition, heart, liver, kidney and steak
from a supermarket should be OK. If you can get some, a fresh placenta
is good for HE staining with a good mixture of elements, human or
animal makes
It would be a shame to get discouraged now after all the time you have already
put into histology. If you still want to work in histology, I might suggest you
try to have a conversation with a manager, supervisor or lead tech and see if
they are willing to support you. Tell them you want to
Alpha Histotech-
I'll put in my few words even though I'm not active anymore and possibly from
different perspective. But also using a few assumptions and if my assumptions
are wrong then the rest of what I say is probably meaningless. Not IDíng your
e-mail address but if you've worked 3
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