[Histonet] Storage of 10% NB formalin

2014-06-11 Thread Barnes, Carolyn K. RICVAMC
My lab is having a discussion about the storage location of our prefilled NB 10% formalin in a stock room. These containers haven't been used or opened. Does anyone know what the current recommendations are for the safe storage of NB 10% formalin? I seem to recall something about an allowed total

[Histonet] Microwave Processor

2014-06-11 Thread Bustamante, Lin
I am looking to buy a NEW Microwave tissue processor. Could you please give me your pro and con for instruments available in your lab? Price is a big issue, if anyone knows about a cheap but very reliable I would like to hear about it PLEASE! Thank you very much. Lin. Lin S. Bustamante, B.S.,

[Histonet] AUTO: Bruce Palmatier is out of the office (returning 06/13/2014)

2014-06-11 Thread Bruce_Palmatier
I am out of the office until 06/13/2014. I will be out of the office from June 10 through June 13. I will have limited access to email but will be responding as possible in the evening. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmat...@vwr.com mobile:

Re: [Histonet] Microwave Processor

2014-06-11 Thread Rene J Buesa
Look at the options presented by Milestone René J.  On Wednesday, June 11, 2014 9:56 AM, Bustamante, Lin lbustama...@cvm.tamu.edu wrote: I am looking to buy a NEW Microwave tissue processor. Could you please give me your pro and con for instruments available in your lab? Price is a big

RE: [Histonet] Re: Request for Non-fluorescent Sealant

2014-06-11 Thread Michael Kent
John, I was recommended and have used black nail polish for FISH applications without background. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paul Scott Sent: Tuesday, June 10, 2014 1:43 PM To:

[Histonet] Aperio slide scanner

2014-06-11 Thread Jan Shivers
My department may purchase an Aperio slide scanner in the near future. My question is - how tech savvy does one need to be to operate the device? I have staffing concerns and the amount of training time involved. Thanks in advance. -- Jan Shivers IHC/Histology Section Head Pathology Teaching

RE: [Histonet] Aperio slide scanner

2014-06-11 Thread Elizabeth Chlipala
Jan They are not that difficult to operate but you do need person with some histology training scanning and QC'ing the slides and images. The need to understand initially if the section is good enough to scan, we QC our slides prior to placing them on the scanner, ones that have sectioning

RE: [Histonet] Aperio slide scanner

2014-06-11 Thread Bea DeBrosse-Serra
Jan, We like ours a lot. And I agree with all that Liz mentioned. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Aperio slide scanner

2014-06-11 Thread Connolly, Brett M
I will echo Liz's comments. I occasionally scan slides with an older Scanscope XT. It is worth the effort to clean and QC slides for artifacts as Liz points out as well as placing the section in the middle of the slide. Actual scanning is quite easy, each initial 'snapshot' is reviewed, AOI is

RE: [Histonet] Aperio slide scanner

2014-06-11 Thread Elizabeth Chlipala
We load and scan the same way Brett does, it works quite well and we rarely have to rescan anything. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com

[Histonet] Dako

2014-06-11 Thread Deloris Carter
Any feedback out there on the Dako Omnis? Deloris Carter HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] non-fluorescent tissue marking dyes

2014-06-11 Thread Michael Giacomelli
I am working on a pathology study involving two photon microscopy of pathology samples. For our study we need to be able to mark the surface aspects of our tissue with dye prior to histology processing and multiphoton imaging. Unfortunately, most of the standard tissue marking dyes we use have