Morning All!
I have heard for years the general problems with using recycled alcohols on HE
stainers, but do the same problems occur when using recycled xylene?
Thanks!
Jeanine H. Sanders
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
404-639-3590
j...@cdc.gov
Does anyone in the United States provide service for the Ventana Coreo Slide
Scanner other than Ventana?
Many Thanks,
Susan
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Histonet@lists.utsouthwestern.edu
While I can agree that recycling alcohol and xylene is both environmentally and
economically advantageous, technically it's awful. There's no way to make used
alcohol and xylene as pure as it was originally. There's also the issue of
fumes from recycling a solvent. The company I had used
We have never had an issue with either our recycled xylene or alcohol that was
not self inflicted. When our system is running there are no fumes.
Tom Podawiltz HT (ASCP)
Histology Section Head
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220
-Original Message-
From:
Maybe your pathologists aren't as fussy as the pathologists I worked with at
the time.
Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508
From:
I agree with Tom. With the exception of self-inflicted issues we also have not
had any issues with recycling our reagents. We check each batch as it is
recycled.
We also don't have a problem with fumes. (And our pathologists are fussy)
-Original Message-
From:
We routinely recycle both our alcohols and xylenes. They are checked for
purity and with the alcohol the extra step of ensuring that we are
getting the correct percentage (95%) recovered. We have never had any
issues in any of our processors or stainers since using recycled
reagents. We also have
They are very fussy as you put it. However, I am even more anal than they are
about our work. We do QA checks both before and after we recycle.
Tom Podawiltz HT (ASCP)
Histology Section Head
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220
-Original Message-
From: Barbara
I have used recycled xylene since the mid-80s and the only problem is that it
is purer than new xylene and can make biopsies crispy. (The isomers get
distilled out.) We use new xylene on the biopsy processor. The recycler is in
our lab and there are no fumes at all.
Surely does save money.
Hi Joyce,
Absolutely agree with recycling concept, value, money saved and no fumes in lab
(if using newer models) and if used properly. I've always been curious about
the concept of a lab recycler making xylene purer by distilling out isomers.
Which unit do you have? meta-xylene is in great
We only recycle xylene which we've tested purity and was close to 98-99% and
is used in both HE staining and processing with no issue. I've done 8 hour
xylene vapor monitoring in our recycling room, while recycling was
occurring, and the results were 6.0ppm where the OSHA exposure limit is
A single permanent, federal job opening for a research Histotechnologist/Lab
Manager is now open immediately for application.
The position is within the Agricultural Research Service, USDA at the Plum
Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY
or Old
This might be a little off the topic: We recently had the enjoyment of
undergoing a day-long EPA inspection and we were issued a warning of
practicing waste management without proper permits when they saw our
recycler. Has anyone else ever been hassled by the EPA regarding this?
Jim
Hmm. Maybe the company who manufactured our recycler went out of business or
we got a lemon. As soon as we abandoned recycling and went back to new
reagents, the stains and processing were perfect.
Go figure.
Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
Hi - there are a couple of instruments that automate ISH and RISH. Our
lab was a beta site for BioCare's Oncore and we loved it. It has an
open platform and is very user friendly. The Oncore is a very compact
instrument and the maintenance was easy and minimal. All of the techs
here liked it.
Haven't heard this one till now!
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's
Hospital and
Well..that's a new one!!!
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com
-Original Message-
From:
Gotta go with what works!!
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's
Hospital and is
This topic was discussed at a meeting somewhere that I attended and here with
our safety staff. The work around that we do here to conform to waste disposal
regulations here in Ca. is that xylene for recycling is not classified as
waste, so it does not enter the waste stream. Since you are
I used this exact argument in my defense (as suggested by the manufacturer
of my recycler) and the EPA responded with the following:
You should consider discussing your recycling activity with MassDEP (the
state agency). Every state has unique interpretations on when a recycling
license or
My condolences, Jim. Yes, that happened to me in my former place of employment.
It was not fun.
To the companies who provide recycling equipment, it would behoove you to be
aware of federal guidelines and how it might not only affect your ability to
sell new units, but also how it affects
Do you know where there might be a reference for….”How long can a specimen be
in saline until it should be placed in formalin or other fixative?”
Thank you
Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA 52001
NOTICE: This email may contain legally privileged
The answer to that question will depend on what you are going to do with the
tissue (HE, IHC, or molecular testing)?
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80
Hi Nancy,
I found this paper (unfortunately I don't have access to the full article) that
may be of help; they looked at various time points for holding the tissue in
saline before being fixed in formalin.
http://www.ncbi.nlm.nih.gov/pubmed/24822006
J Maxillofac Oral Surg. 2014
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