[Histonet] THICK AND THIN SECTIONS ?

If you are using one of the following Microtomes and the advance mechanism is worn out. (too much play) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Mic

[Histonet] IHC for plague and tularemia

Hi everyone, I would love to hear from anyone who is running plague and/or tularemia IHC. If you have a protocol you are willing to share and/or vendor information it would be greatly appreciated. Thank you in advance! Carole Johnson, HT(ASCP)cm Histopathology Section Supervisor New Mexico Depa

[Histonet] Can I use no water benzoyl peroxide from PolySciences in my MMA mixture?

I try to avoid CaCl drying step in making MMA infiltration and embedding solutions. Please help me MMA experts. Many lab use benzoyl peroxide which has 30% water in it. And go through CaCl afterwards. Another question, why some lab use Butanol and toluene before coverslip? Why use Cellosolve to

[Histonet] Re: IHC Validation

Thank you to everyone who sent a template. This is very helpful. I do need clarfication on the 10 cases that are required for validation. Do you include the 10 cases on this same form, or do you have something separate for that? These look ideal for optimization. From what I have read online,

[Histonet] IHC Validation

Can you forward any templates to me as well? Thanks! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leslie Graves Sent: Tuesday, July 15, 2014 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histo

Re: [Histonet] Re: IHC Validation

I document all of the worksheet process¹ and then I create a ³Validation Report² where my technical director both reviews all slides and signs this report. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 7/

Re: [Histonet] Image uploading

Hi Carl, I have sent you a username and password to be able to edit your image posts. Let me know if you have any problems or suggestions. You have definitely posted some great looking images. Best Regards, Marvin Hanna On 07/12/2014 03:17 PM, Hobbs, Carl wrote: I make mistakes when I uplo

[Histonet] doing histology in an academic research setting

Hi everyone, I have been doing histology professionally for the past 5 years in an academic research lab setting (and an additional 5 years for my own grad studies). I take pride in the samples I have processed, cut, stained, and I love being able to troubleshoot any challenges or questions that

[Histonet] Antibodies to fungus

I think I asked this question a few years ago, but I'm going to ask again DAKO used to sell monoclonal antibodies to Aspergillus (clone Mab-WF-AF-1) and Rhizomucor (clone Mab-WSSA-RA-1). Unfortunately, they discontinued these reagents several years ago. Does anyone know if these anti

[Histonet] Microtome calibration

Hi Everyone, During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. Thank, Denise M. Long, MS, HTL (ASCP), QIHC University of Connec

[Histonet] Control block validation requirements

Same inspectors advised our special stain and IHC controls should have at least two if not three different validation means (PCR, micro, serology, etc.) Anyone out there actually doing this? Just so you know, I've spoken with a few suppliers on control slides and they do not do this type of valid

[Histonet] ER+/PR- in elderly patients

I was wondering if anyone is seeing a trend in ER+/PR- tumors more frequent in older patients? If so, do you have any information regarding this? Thanks~ Brandy Burnett, HTL ~~ This email and any files transmitted with it ar

Re: [Histonet] Microtome calibration

Hi Denise, Check with the company that does your PM service. This may be something that is already done during preventative maintenance. On Jul 15, 2014, at 3:02 PM, "Long, Denise" wrote: > Hi Everyone, > During a recent inspection we were asked by the inspection team to start > calibrating

Re: [Histonet] Microtome calibration

We do ours by hand? You mean line them up or PM? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 7/15/14, 1:55 PM, "Ronda Mire" wrote: >Hi Denise, > >Check with the company that does your PM service. Thi

Re: [Histonet] Control block validation requirements

Denise, When selecting infectious agent positive controls, I also select cases which have been shown to be positive by other testing means (EM, Bacteriology, Virology, PCR). In addition, I run a cross-reactivity validation with my antibody on other infectious agent positive control slides to make

RE: [Histonet] Microtome calibration

Could they have meant an aligner - something like this? http://www.newcomersupply.com/products/microtome-cryostat-aligners?page=M#1130 Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road At

[Histonet] Temp full time job in SW WA

We have a temporary full time position to fill. We are looking for a ASCP certified Histotech that can work from the beginning of September to the end of December/beginning of January. This would be full time coverage for a maternity leave. Hours 6-2:30. Qualifications: ASCP certified Histote