Re: [Histonet] Xylene Substitute Protocols

We have found that Permount works best with the aliphatic hydrocarbons. Sent from my iPad > On Jul 24, 2014, at 10:01 AM, "Hilliard, Dawud Deshawn" wrote: > > Hello all, > > Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type fo

RE: [Histonet] Automated stainer for H&E and Pap

Hi Brandi, We don't do the PAP stain, but do have multiple stains programmed into our Leica ST5020. It can easily handle both stain programs with switching out containers. Regards, Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.u

Re: [Histonet] Automated stainer for H&E and Pap

Actually u would have too have 3 set ups since none can share anything. Sent from my iPhone > On Jul 24, 2014, at 3:20 PM, Brandi wrote: > > I am shopping for a new automated stainer and would prefer one that I can do > my H&E stain as well as my PAP (GY AND NON-GY) stains on. I know that I'

[Histonet] Automated stainer for H&E and Pap

I am shopping for a new automated stainer and would prefer one that I can do my H&E stain as well as my PAP (GY AND NON-GY) stains on. I know that I'm asking for a lot and will have to purchase extra containers to switch back and forth. I'm most interested in the Leica 5020 & Gemini. I would gre

RE: [Histonet] problems Sllde Mate print

We use Thin Prep slides for our cytology and have had trouble with the slides smearing. Very frustrating. __ Lynn M. O'Donnell, CT (ASCP), MHA Technical Specialist, Cytology Danbury Hospital 203-739-6704 Email: lynn.o'donn...@wchn.org -Original Message- Fro

RE: [Histonet] problems Sllde Mate print

Same here for printing staying on. We use Thermo ColorFrost, slides have no problems. It may depend on the finish the manufacturer uses for the paint on the slides. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwe

Re: [Histonet] Midwest Lab Equipment

Haven't heard of them and no experience with them.   But I have dealt with IMEB out of San Diego when setting up labs. Excellent customer service in both buying and selling.  I usually call on Margaret or Charlie. Wanda >  ---Original Message--- >  From: Mark Pawlowski >  To: histonet@l

RE: [Histonet] problems Sllde Mate print

Gudrun, We have 2 slide mates and we run our slides through all reagents and do not have the issue you describe. The only issue we experienced was poor print quality due to the type of paint on the frosted end of the slides. I recently switched to Leica's charged slide that is designed for Th

[Histonet] RE: Xylene Substitute Protocols

Richard Allen mounting media REF 4111. >>> Shannon Kleiner 7/24/2014 12:59 PM >>> Formula 83 in first three stations and last four stations on the Leica XL stainer. >>> "Wineman, Terra" 7/24/2014 12:06 PM >>> We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mountin

[Histonet] Midwest Lab Equipment

Hello, Has anyone had an experience with this company buying or selling used lab equipment? Mark The information contained in this transmission contains privileged and confidential information. It is intended only for the use of the person named above. If you are not the intended recipi

[Histonet] RE: Xylene Substitute Protocols

Formula 83 in first three stations and last four stations on the Leica XL stainer. >>> "Wineman, Terra" 7/24/2014 12:06 PM >>> We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mounting type Terra Wineman, HTL(ASCP)cm Novus International -Original Message- Fro

[Histonet] problems Sllde Mate print

Dear histonetters, we have problems with the stability of the print of our slide mates (Thermo). We use regular slides with frosted end. The print usually looks good and stable at the time of printing. But after staining the print comes off like peeled skin. Very easy to remove. We take care

[Histonet] RE: Xylene Substitute Protocols

We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mounting type Terra Wineman, HTL(ASCP)cm Novus International -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud Deshaw

[Histonet] Xylene Substitute Protocols

Hello all, Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type for coverslipping? We have tried a few, but without much success. We are currently trying Leica's Sub-X, also without much success. I greatly appreciate the insight

[Histonet] FT Day-Shift Histotech Openings - Certification not Required!

Histonetters, University Health in Shreveport, LA is currently offering two full-time histotechnologist positions. Openings are for day shift Monday-Friday with opportunity for weekend/holiday call pay. Pay and benefits are regionally competitive. Applicants have the opportunity to acquire sp

[Histonet] Mouse on Mouse Staining

Good Morning, I have avoided for the last few years doing IHC with mouse on mouse antibodies, but have been requested to do so at this time. I have two antibodies, HDAC1 and Nitrotyrosine from Santa Cruz that I am working up, however the staining pattern in my tissue does not seem to be reveali