[Histonet] whole slide scanners

2014-08-05 Thread Kalleberg, Kristopher
Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Desmoglein-3

2014-08-05 Thread Bitting, Angela K.
Ventana Benchmark Ultra users: Where are you buying your Desmoglein-3? I need an IVD product. Thanks in advance, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for

RE: [Histonet] whole slide scanners

2014-08-05 Thread Jamal
Did you find out about Aperio ePathology, it’s the most famous technology for slide imaging You can visit the link below http://www.leicabiosystems.com/pathology-imaging/epathology/ Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories

[Histonet] Exakt tech

2014-08-05 Thread Ronda Mire
Hello Netters, Anyone in Histoland know of a good Exakt tech? It is a very specialized skill and I need to identify some individuals that have this ability. Thanks for your input Ronda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RE: whole slide scanners

2014-08-05 Thread James Watson
The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only

[Histonet] RE: whole slide scanners

2014-08-05 Thread Elizabeth Chlipala
I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be

[Histonet] control slides

2014-08-05 Thread Nancy Schmitt
Good Morning- We keep a control tissue log that documents where the tissue comes from, what stains it is used and has been tested for, when each block is put into use and exhausted and pathologist of record. When a block goes into use, do you mark on each slide the date it was cut (for those

[Histonet] Protocol for decalcifying and processing large bone section.

2014-08-05 Thread Clough, Bret
Hi everyone, I was hoping someone on histonet would being willing to help me by either sharing their protocol or advising me on decalcifying and processing large bone sections. The bone sections are from the femural head of sheep measuring roughly 1cm x 1 1/2 cm cube. Currently I’ve been

Re: [Histonet] control slides

2014-08-05 Thread Sue
We actually just stared keeping a log for special stains, always for IHC.  Our rule of thumb is that an IHC control slide cannot be oler than 6 months,  although we go through most of them much faster.  For the basic IHC, tonsil, fallopian tube etc we do not log the case information for IHC

Re: [Histonet] Protocol for decalcifying and processing large bone section.

2014-08-05 Thread William J. O'Connor III
Is there a need to decalcify the femoral head intact? We typically cut large, unfixed veterinary bones with an Exakt band saw into 5 mm slices prior to fixation and decalcification. This ensures the bones are well fixed prior to decalcification. We decalcify the selected slices for about

Re: [Histonet] Protocol for decalcifying and processing large bone section.

2014-08-05 Thread Rene J Buesa
As a general rule EDTA is used for the gentle decalcification of small or delicate bones, such as bone marrow samples. For large and dense bones, like the one your are trying to decalcify, you should use a strong acid. In order to prevent having to prepare the decalcifying solution I always

[Histonet] RE: whole slide scanners

2014-08-05 Thread Bea DeBrosse-Serra
I totally agree with both of you. We upgraded our Aperio (Leica) ScanScope XT to the new AT, which holds 400 slides and is much faster than the XT. Both can also scan 2x3 slides manually. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct.

RE: [Histonet] Protocol for decalcifying and processing large bone section.

2014-08-05 Thread DiCarlo, Margaret
I use 10% formic acid for large human bones but I don't think your bone sections are that large. Peggy DiCarlo Ortho Bone Lab BGMC 100 High St. Buffalo, NY 14203 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

AW: [Histonet] RE: whole slide scanners

2014-08-05 Thread Gudrun Lang
Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014

RE: [Histonet] RE: whole slide scanners

2014-08-05 Thread Elizabeth Chlipala
We are a research based histology lab so we use scanning for several reasons: 1. We scan slides for clients so they can review their images remotely and capture snapshot images, annotate, etc- study sizes may range from 10 to 100's of slides 2. We scan all of our slides for IHC protocol

[Histonet] Listservers in microbiology

2014-08-05 Thread Jorge A. Santiago-Blay
Hello: I have found Histonet quite useful and the members with whom I have communicated helpful. Can someone recommend listservers with similar qualities that emphasize microbiology? If yes, please feel free to email at blayjo...@gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD

AW: [Histonet] RE: whole slide scanners

2014-08-05 Thread Gudrun Lang
Does anyone out there use digital pathology for routine diagnostics? Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Dienstag, 05. August 2014 19:13 An: 'Elizabeth

[Histonet] Procedures and Policy manual needed

2014-08-05 Thread Dr. Max Mirot
I am attempting to re-write our procedure and policy manual. Our scope of service is routine anatomic pathology/histology and Non Gyn cytology. Right now the lab is CLIA certified. Anyone care to share their policy and procedure manual? I would like to look at a couple of nicely done manuals to

[Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770

2014-08-05 Thread Cooper, Brian
I've never been asked by a CAP auditor either, but our internal inspectors used to inquire about this in a previous lab all the time. We use Bromocresol Purple as an indicator here, and we have a simple log sheet wherein our crew just tests one piece of cleaned glassware each day. They write

RE: [Histonet] RE: whole slide scanners

2014-08-05 Thread Morken, Timothy
We are setting up Philips high speed slide scanners to scan frozen sections for remote Dx. One critical aspect is to get coverslip medium that dries fast... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA

RE: [Histonet] Listservers in microbiology

2014-08-05 Thread Cartun, Richard
Our Director of Microbiology gave me this information: Yes there is an excellent Listserver in microbiology but it is by invitation and requires ASM membership. Clinmicronetgroup. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs

RE: [Histonet] RE: whole slide scanners

2014-08-05 Thread James Watson
We are pharma research, about 40-45% of our slides are fluorescent. With 1 scanner. 201317,096 slides scanned 201320,501 slides scanned James Watson HT  ASCP GNF  Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 

RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770

2014-08-05 Thread Joelle Weaver
Do the same as Brian. It is not that involved really, and its not worth getting a deficiency over. Joelle Weaver MAOM, HTL (ASCP) QIHC From: bcoo...@chla.usc.edu To: lcolb...@pathmdlabs.com; histonet@lists.utsouthwestern.edu Date: Tue, 5 Aug 2014 19:07:05 + CC: