Dear Histonet,
I am having a major problem with bubbles in my mounting media. I am currently
using the Leica coverslipper and Cellpath mounting media (Phalate free).
Micro-bubbles are observed on the slides after sometime of coverslipping. In
fact, when I checked the media, I found lots of
Dear Histonet,
I am having a major problem with bubbles in my mounting media. I am currently
using the Leica coverslipper and Cellpath mounting media (Phalate free).
Micro-bubbles are observed on the slides after sometime of coverslipping. In
fact, when I checked the media, I found lots of
Hi Melissa
Try to fill the tube of mounting media some hours before use.
I use to fill it in the end of the day and in the next day the micro bubbles
will be completely disappeared.
Best Regards,
Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg
Medical Laboratories |
Do you know what the dilution is from Biocare, and how many tests per vial?
From: Weems, Joyce K. [joyce.we...@emoryhealthcare.org]
Sent: Wednesday, August 06, 2014 5:02 PM
To: 'Ronda Mire'; Burnett, Brandy
Cc: histonet@lists.utsouthwestern.edu;
Try adding xylene to the mounting medium to thin it. About 10 ml of xylene to
90 ml of mounting medium should do it.
Then, leave the bottle of mounting medium for about a day or two to allow any
air that may have gotten mixed into to dissipate.
Barbara S. Tibbs
Histology Supervisor
Accurate
Melissa we are having the same problem. We use a different mounting medium. I
will try Barbara's advice and try adding a bit of xylene to the media.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little
What is a good pay wage for a 25yr experience in Histology. Any good
information out there ? Indiana based.
S Kathy Baldwin
Histology/Cytology Supervisor
PH. 812-996-0210, Fax 812-996-0232
sbald...@mhhcc.orgmailto:sbald...@mhhcc.org:)
Christ's healing mission of compassion empowers us to
Is it necessary to run a negative slide per IHC case per CLIA when using
polymer based detection? Does anyone have references?
Thank you,
Sent from my iPhone
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I have a couple of questions to ask where there is no right or wrong answer,
just curious as to the process that other labs use.
1. After processing, how do you determine the order in which to cut and stain
the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours
of
When I worked in clinical labs the order was first the priority cases
(biopsies, bone marrows, etc.) then numerically.
I remember once two physicians brought small biopsies and were in a hurry for
the results, both wanted to be first out and I can still hear myself explaining
to them that there
... and I can still hear myself explaining to them that there could only be
ONE first!
Yes, DAILY!!! Every time someone wants to change things around so they get
theirs first, this is our only defense as to why everyone can't get theirs
first. 8 years of college doesn't seem to help here...
Our cases are cut by priority. And we don't process breasts. (children's
hospital)
Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
We use p16 on the BondMax at a 1:4 dilution. There are 11.5 ml in the container
when received.
Comes from Dako.
Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
Dubuque, IA
*
Message: 12
Date: Thu, 7 Aug 2014 13:06:37 +
1. Our early (small biopsies)processor finishes at 4am. Anything with special
instructions (Cyto blocks, liver bxs, pros bxs, Bone Marrow cases) are done
first. Then just by numerical as they come out of the rack. Late processor
finishes at 0630 and the first done are Breast bxs, sent node
Are you sure Dako has p16?
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Actually comes from Roche..sorry about that.
Nancy Schmitt MLT, HT(ASCP)
Histology Coordinator
NOTICE: This email may contain legally privileged information. The information
is for the use of only the intended recipient(s) even if addressed
incorrectly. If you are not the intended
Currently we have a IHC QC form for every case that we run. It lists dr,
antibody, date, comments, quality results. The HT and pathologist sign off on
the quality. Is this necessary for every case or can we do a daily log for all
cases? This process is a little time consuming. Does anyone have
Sounds like we are all working at the same place! LOL
I hear exactly the same questions.is it something they teach to all
pathology residents
Martha Ward, MT (ASCP) QIHC
Manager
Molecular Diagnostics Lab
Medical Center Boulevard \ Winston-Salem, NC 27157
p 336.716.2109 \ f
Priority driven block order, by specimen type/protocol, defined in SOP ( TAT
for 'stat, ASAP routine)
Fixation is monitored for all tissues, just easier to do all the same, made
entry field in LIS, and tracking log for manual back up for computer down
times. Keep to CAP guidelines for breast,
We only check and document control slide quality and need for repeat due to
control failure.
The pathologist reviewing the slides will send a QC form to us if there is a
problem with case slides. Plus thye can enter a repeat for a poor stain as a
repeat and we can track the number of repeats
How long have you been a registered histotech? 36 years here. You???
Douglas A. Porter, HT (ASCP)
Grossing Technician
IT Coordinator
Cancer Registrar
CAP-Lab, PLC
2508 South Cedar Street
Lansing, MI 48910-3138
517-372-5520 (phone)
517-372-5540
44 years here.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter
Sent: Thursday, August 07, 2014 2:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] On the lighter side...
How
Friends -
One of our metrics we monitor in our lab is a receive to sign out time metric.
We measure the time the specimen comes to the lab to the time the case is
signed out by a pathologist. We normally run on average 31-32 hours per case
for our inpatients (about 1200 cases per month) but
36 years.
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
5830 N Blue Lake Dr. Please note new address!
Norman, OK 73069
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
From: Sullivan, Beatrice bsulli...@virtua.org
To: 'Douglas
39 years for me
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net
Date: Thu, 7 Aug 2014 11:59:44 -0700
From: cont...@excaliburpathology.com
To: histonet@lists.utsouthwestern.edu
46 - but I was 2 when I took the sat for the exam
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint
29. I feel so young...
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu
-Original Message-
From:
We embed and cut and by priority for: 1) bone marrows, 2) biopsies, 3) breast
cases 4) surgicals, 5) placentas, 6) autopsies. RUSH cases for kidney, liver
and heart take priority over all!
Occasionally we will have requests for a specific case to be cut earlier than
the usual priority list
41 years.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org
-Original Message-
From:
I just made 50. Started early.
Pam
- Original Message -
From: Hazel V Horn hor...@archildrens.org
To: Paula Pierce cont...@excaliburpathology.com, Histonet
histonet@lists.utsouthwestern.edu
Sent: Thursday, August 7, 2014 2:09:40 PM
Subject: RE: [Histonet] On the lighter side...
So here at TJUH, we are color coded and all blocks other than routine and
decals are cut in this manner. One tech is assigned rapids (green), another
tech is assigned bone marrow and cell blocks (yellow and blue) and one tech is
assiged out satalite hospital (pink cassettes) All are
41 years also!!
Sharon Beckham
Head, Histotechnology
Stowers Institute for Medical Research
Kansas City, MO
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Thursday, August 07, 2014
Hi everyone,
As I read and was told, formalin pigment is seen as a dark precipitate
under light microscope. I also read that you can see this via polarizing
microscope as it is birifringent. I'm wondering: does it first need to be
seen under the light microscope, and birifringence is just a
13 years
Michaela LeFaivre BS, HTL (ASCP) CM
Molecular Technician IV
Molecular Pathology
Rm 4344 Purple Zone
919-684-4303
919-684-5095
HIPAA Privacy Notification: This message and any accompanying documents are
covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and
34 years. Egads, I'm OLD! When did that happen?
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
Today's Topics:
12. On the lighter side... (Douglas Porter)
Message: 12
Well I'm just a baby.in pathology 6 years...only!
Sent from my iPhone
On Aug 7, 2014, at 3:47 PM, Terri Braud tbr...@holyredeemer.com wrote:
34 years. Egads, I'm OLD! When did that happen?
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
This e-mail may contain confidential, personal and/or health
information(information which may be subject to legal restrictions on use,
retention and/or disclosure) for the sole use of the intended recipient. Any
review or distribution by anyone other than
36 years here
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Thursday, August 07, 2014 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...
36
30years tomorrow.
Lee Ann Baldridge
IUSM
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter
Sent: Thursday, August 07, 2014 2:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] On
Historically, we used LVN (low viscosity nitrocellulose, also marketed as
Celloidin and various other trade names) to embed specimens such as whole
decalcified salmon heads for thick sectioning (30 to 100µm) using sliding
microtomes such as the AO 860.
I used to be able to purchase LVN powder
Well...I appears most of us are right properly fixed. Good to see some
newbies chiming in. This also shows that we need to get/keep more of the
young ones interested in the field.
Douglas A. Porter, HT (ASCP)
Grossing Technician
IT Coordinator
Cancer Registrar
CAP-Lab, PLC
2508 South Cedar
I have been recruiting exclusively in histology for 10 years. ; )
Thanks-Pam
Right Place, Right Time, Right Opportunity.
Pam Barker RELIA Solutions.
rel...@earthlink.net
(407) 353-5070 cell/text
Sent from my Galaxy S®III
div Original message /divdivFrom: Douglas Porter
Technically LVN and celloidin are not quite the same.
LVN (also known as gun cotton) is useful for large specimens -we used to
use this for aborted fetal heads and large bone specimens. It penetrates
much more rapidly that celloidin and is much easier and safer to handle.
Tissues prepared
35 years here. Histology has been very, very good to me! :-)
Mark Turner, Ph.D., HT(ASCP)QIHC
Manager, Histology/IHC
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Thursday,
28 years here
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Howery, Jeff L
Sent: Thursday, August 07, 2014 3:54 PM
To: 'Paula Pierce'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the
34 years for me, but it seems like just yesterday it was 33.999 years.
Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803
-Original Message-
From:
Hello all,
Recently we have been experiencing a problem when running HE slides on our
Gemini stainer (Thermo Shandon Varistain). At the end of the run, small
water droplets or possibly air bubbles are seen on the slides after taking
the slides out of xylene. If they are coverslipped, a white haze
Hi Julie,
We have the Gemini stainer and have noticed that water gets trapped in the top
of the slide rack. Make sure the level of your dehydrating alcohols after the
eosin are high enough to cover the top of the rack, and then make sure your
xylene level is high enough to get rid of all the
Celebrated 1yr HTL in July
Terra Wineman, HTL (ASCP)CM
Research Biologist, Nutritional Physiology
636-926-7476 phone
terra.wine...@novusint.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly,
16 years!!!
Kathy Machado, HTL
Danville Regional Medical Center
Danville, VA
kathy.mach...@lpnt.netmailto:kathy.mach...@lpnt.net
434-799-3867
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15 Years and still going strong :)
Vincent Rivera, HT (ASCP), QIHC, QLS
Histopathology Supervisor
West Dermatology Pathology Laboratory
vriv...@westderm.com
714-924-7240 (Lab)
714-390-0906 (Cell)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
44 years here
On 8/7/14 1:26 PM, histonet-requ...@lists.utsouthwestern.edu
histonet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
36
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
kathy.mach...@lpnt.net
Sent: Thursday, August 07, 2014 4:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] re: on the light er side...
16
31 years
Jennifer MacDonald
From: Douglas Porter doug.por...@caplab.org
To: histonet@lists.utsouthwestern.edu
Date: 08/07/2014 11:36 AM
Subject:[Histonet] On the lighter side...
Sent by:histonet-boun...@lists.utsouthwestern.edu
How long have you been a registered
Registered 4 years
Doing histology 30 years.
On Thu, Aug 7, 2014 at 5:19 PM, Jennifer MacDonald jmacdon...@mtsac.edu
wrote:
31 years
Jennifer MacDonald
From: Douglas Porter doug.por...@caplab.org
To: histonet@lists.utsouthwestern.edu
Date: 08/07/2014 11:36 AM
Subject:
28 years
Rick Carter
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Thursday, August 7, 2014 2:20 PM
To: Douglas Porter
Cc: histonet@lists.utsouthwestern.edu;
10 years certified, 12 total! :)
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15 years and over 10 certified HTL.
Claire Ingles
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Richard A. Carter
[rcar...@cancer-test.com]
Sent: Thursday, August 07, 2014 4:36 PM
To: 'Jennifer
49 years and 45 years certified as HT and 22 as HTL
Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
Email: aalli...@montereygi.com
Tele: (831) 375-3577 X117
On Aug 7, 2014, at 3:39 PM, Ingles Claire
Dear Hazel and Barbara,
Thank you for your feedback. Wouldn't adding too much xylene result in drying
effects post-coverslipping?
Best thanks and regards,
Melissa
-Original Message-
From: Horn, Hazel V [mailto:hor...@archildrens.org]
Sent: Thursday, 7 August, 2014 10:10 PM
To:
I've been registered since 1969. This thread is interesting and I'd like to
see what those of us Who Have Been in this Game so Long have as registry
numbers. Mine is 04958 (I think...). How many of you can beat that? But
who's counting? I retired 2.5 years ago but I'll always be a
I must be getting old - my ASCP number is 04258, not 04958 (or whatever number
I first wrote down)... I hate it when that happens!
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