Re: [Histonet] Traveling Histotech

We used TechForce3 to cover my maternity leave and were happy with the services. Sent from my iPhone > On Aug 20, 2014, at 1:07 PM, michele carr wrote: > > Hi everyone, I was wondering if you could recommend a reputable agency for > traveling histotech. > Thanks > __

[Histonet] Traveling Histotech

Hi everyone, I was wondering if you could recommend a reputable agency for traveling histotech. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] frozen sections

Hi All, Thanks for all of your help with the slippery floors. I was also wondering which personnel labs are using for performing frozen sections? Lab Assistants? Histotechs? What are your minimal requirements? Do you classify frozen sections as high complexity testing? Thank you, Lacie Lac

[Histonet] IHC on free floating slices of brain

Dear Walter, In order to cut the tissue on the microtome, you need to first fixed the brain tissue (in rodents, normally, we perfused it using 4% PFA or 10% formalin), followed by post-fixation for a couple hours depending on the antibody of interest. Prior to cutting the tissue on the microtome,

[Histonet] RE: great bone saw

We LOVE the IMEB table top band saw for bone. It has a tiny footprint, easy to clean and operate, and it cuts incredible slices without any damage to the bone. It can use a regular blade for standard everyday bone sectioning, or a diamond blade for super delicate work. And no, I don't get any com

[Histonet] Vacuum Assisted Breast Biopsies

For needle core breast biopsies, we cut several unstained slides - 3 levels and 2 unstained serials at each level. For vacuum assisted breast biopsies, which have much more tissue and many more blocks, we are finding that we are wasting a lot of slides cutting so many unstained. However, just

[Histonet] RE: IHC on free floating slices of brain

Hello Walter, Many years ago I did Timm's Silver stain on free-floating rat brain slices. The rats were perfused before removal and freezing of the brain on dry ice. I then would cut slices in a cryostat and float the sections in saline until ready to stain. I would imagine that it might be a

[Histonet] Coverslipper

Dear colleagues Do any one have experience or information about Leica Coverslipper ST5010-CV5030 Workstation stainer. I am planning to add a new autostainer with coverslipper but I am confused between this brand and Tissue-Tek R Prisma R & Coverslipper. Best Regards, Jamal M. Al