Another Hallmark is the Superficial Vascular Plexus of
the Skin.
You can do Muscle Actin ( HHF – 35 ) or CD 34 to
remark the vessels.
Link to a Picture
http://biologiedelapeau.fr/spip.php?page=forumid_article=21lang=en
This Superficial Vascular Plexus allows separating
Invasive Melanoma
Embedding:
I use a large, solid aluminum block that I keep cool in my cryostat. It's
approx. 9 in (length) x 4 in (width) x 3 in (depth). I just stick the epidermal
edge to the block and flatten it out so all margins are in contact with the
freezing surface. Drop some freezing medium on it and
Happy Friday Histonetters!
I am working on a histology platform in a research center and someone came
to me last week and asked to cut blocs of resin (JB-4 resin) on the
microtome. I never cut anything else than paraffin, so I was wondering if
some of you had advices for me?
They never did it
Veronique,
May I ask what type of specimen is embedded into the JB-4 resin? Nevertheless,
you should be able to cut these blocks using a tungsten-carbide knife. While
there are a few vendors out there that sell these knives, in my laboratory I
personally use knives re-sharpened by Delaware
I've only cut resin with a glass or diamond knife in an ultramicrotome. If you
are attempting to do it in a regular microtome, you would need a special blade
holder. I don't know if any microtome manufactures make glass knife holders.
You make the glass blades yourself using special glass.
Yes brightinstruments, com make glass knife holder and tungsten carbide tipped
knives for microtomes,
KR,Alan Bright
Sent from my iPhone
On 12 Sep 2014, at 15:49, Keyser Gerald T gkey...@uwhealth.org wrote:
I've only cut resin with a glass or diamond knife in an ultramicrotome. If
You can use Smooth Muscle Actin, Calponin, Myosin and CD34 to stain the vessels.
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Cesar Francisco Romero
[chesar...@hotmail.com]
Sent: Friday,
Happy Friday Histopeeps!
I am looking for opinions on tape coverslippers. I currently have a Leica
CV5030 and while it is only 5 years young, it can be a bit petulant. I
would like to find something refurbished, but haven't had any luck. We are
a low volume lab averaging 100 slides/day. Any
Dear Histonet Listers:
Other than nigrosin stain or eosin stain, do you know what other stains are
good for negative staining of bacteria. While I am aware of the principles
of negative staining, I would like to know what have been used successfully
for this simple technique.
If you know of
We have had a Sakura tape coverslipper for 17 years with hardly any problems.
The slide storage quality is excellent.
Lynn M Burton
Histology
Animal Disease Lab
Galesburg, Il
309-344-2451
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Would anyone have a protocol for C-Myc using the Ventana Benchmark Ultra or
XT? Thanks in advance for any information you can provide.
Rae Ann Staskiewicz
UnityPoint Health Methodist
Peoria, IL
309-672-5994
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We do. I'll email Tuesday when I'm back at work.
Linda A. Sebree
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Rae Staskiewicz
[raest...@grics.net]
Sent: Friday, September 12, 2014 3:33 PM
To:
Hello All,
We have a Leica histoembedder that is on the fritz. It works well and is quite
convenient as long as it decides to turn on. Leica is insanely expensive for
any service call and, depending on the price of the embedder, it could be
cheaper to buy a new one.
I am writing to find out
Does anybody have any paraffin embedded Metamophosed Axolotl Heads?
André Charbonneau
M.Sc. Candidate
Faculty of Dentistry
McGill University
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I used to have a triangle glass knife holder insert for my Leica microtome or I
would use the tungsten carbide knives. It depends on what you are cutting. if
it is calcified bone the glass knives scratch too much and they are only 1/2
inch wide so you have to cut smaller soft tissues with
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