Good Morning,
I was wondering if anyone uses the Merifluor DFA kit for Pneumocystis and if so
where can you buy controls for it? We have had no positive cases to make our
own cytospin controls.
Thank you,
Jessica Piche, HT(ASCP)
Waterbury Hospital
CONFIDENTIALITY NOTICE: This email and any
Hello folks,
I am wondering which mounting medium für IF you are using in your daily
routine.
We are using Prolong antifade gold, but it is really really really
expensive and I was wondering if there is a good alternative. I just
ordered a sample of the Dako Fluorescence Mounting Medium so we can
Hi Christina,
We use DPX. It has worked very well for us. I am not sure about the cost
though, but anything bought in kits can get really pricy. DPX comes in 500 ml.
Claudia N. Segovia
Senior Neurohistologist
Antibody Specialist
NeuroScience Associates
10915 Lake Ridge Drive
Knoxville, TN
If you are using one of the following Microtomes and the advance mechanism
is worn out.
( too much spindle play )
REICHERT/JUNG 2030
LEICARM 2125
LEICA 2030 Biocut
LEICA/JUNG2035
LEICA - CM 1850 Cryostat
SAKURA SRM 200
You could be faced with
If you are using one of the following Microtomes and the advance mechanism
is worn out.
( too much spindle play)
REICHERT/JUNG 2030
LEICA RM 2125
LEICA 2030 Biocut
LEICA/JUNG2035
LEICA -CM 1800 Cryostat
SAKURA - SRM 200
You could be faced with
Good morning!
Open position-- a grosser for a new derm lab. It's in the Northeast right on
the Atlantic Ocean Beautiful location, good hours. You do not have to be a
registered histotech: CLIA '88 gross-qualified is required. (Not sure what that
is--call me!)
All inquiries welcome.
Other
We use Vectashield Mounting Medium, catalog# H-1000, Vector Laboratories, 10
ml. My pathologists absolutely love it. It really works for retarding
photobleaching and really preserves fluorescence.
Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
one...@wvuhealthcare.commailto:one...@wvuhealthcare.com
We have used a hardmount Thermo Immumount Cat# 9990402. It is the only
hardmount we tested that gave good focus using the NanoZoomer scanner. No need
to seal with nail polish, saving a lot of time. We just re-scanned slides
that were stained 6 months ago and still had great fluorescence.
Hi Christina,
Espada et al (2005) have shown that fluorochromes are very well preserved after
dehydration (or simply air drying) followed by permanent mounting in
non-aqueous synthetic media, particularly DePeX. They also found that
fluorescence had not faded after one year of storage at room
