Here are the descriptions per the 2014 CPT and HCPCS books:
88342 – Immunohistochemistry or immunocytochemistry, each separately
identifiable antibody per block, cytologic preparation, or hematologic smear;
first separately identifiable antibody per slide
88343 – Each additional separately
What is the minimum time recommended for fixing routine endoscopy biopsies
before placing them on a short bx run program.
Thank you for your help
Fawn
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confidential or
Our institution is billing IHC identical to what Joyce stated.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
Message: 3
Date: Wed, 8 Oct 2014 14:44:54 -0500
From: Horn, Hazel
Hi Histonetters!!
I hope you are enjoying a beautiful day. How bad could it be? Its October,
Its Football season, the weather is cooling down and Its just one more day
til the Weekend!! (and for all you walker hunters out there its just 3
more days till the season premiere)
I am also in a
Our new Pathologist would like to see more yellow in the background of our Gram
stain. We use picric acid-acetone currently, which looks more pink than yellow
most times. So I'm wondering if a method using a Tartrazine counterstain would
be better.
Any thoughts?
IMPORTANT WARNING: The
Dare I ask?! Are any Pathology labs discussing what to do with
specimens/precautions, etc. regarding a person with a potential Ebola infection?
Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph 610-378-2635
fax 610-898-5871
Handle with universal precautions as always is our emphasis.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of
We use the McDonald's Gram Stain kit from American Master Tech, which employs
the use of a Modified Tartrazine counterstain. Both our docs and techs love
our Gram stains . . .
Here's the link to the product info.
http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStylesitem=KTMGS
Have it delivered in formalin and let it fix completely.
Formalin fixation eliminates the biologic hazard associated with transporting
samples containing infectious EBO virus, Thus, formalin
fixation provides an adequate noninfectious specimen for EHF diagnosis,
I did my postdoc research at USAMRIID on Ebola virus pathogenesis. Our standard
fixation protocol for Ebola, Lassa and other BSL4 pathogens was to let them fix
in formalin for a minimum of 30 days. The containers of fixed tissue were not
allowed out of the P4 containment lab until the 30 day
If formalin didn't kill CJD, what did you use? Just curious.
Emily
By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward.
-Chuck Palahniuk, Haunted
On Thu, Oct 9, 2014
Back in the 80 NIH did extensive studies on CJD, their protocol requires 24
hours in formalin, 24 hours in formic acid, followed by 48 hours in formalin,
then paraffin processing. Worked with CJD at Frederick Cancer Research in
collaboration with the group from NIH. If I find the
Actually it has been proven that formalin will inactivate Ebola, as well as
other hemorrhagic fever viruses.
Brett
Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803
Correction, due to years of formalin abuse, it was 1 hour in concentrated
formic acid. We then processed and cut them with all other cases. A few cases
slipped through with just formalin fixation and additional precautions needed
to be taken. That said the easiest and primary route of
We tried very hard not to do CJD cases if we could help it, as I recall any
suspect cases were sent to Case Western Reserve, i worked with someone there
who developed a protocol using strong formic acid, it was amazing how good the
tissue preservation was using formic acid as a fixative. I
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