Oh ok that makes sense. Thanks
-Original Message-
From: Smith, Allen [mailto:asm...@barry.edu]
Sent: Monday, January 05, 2015 4:59 PM
To: Roy, Ryan
Cc: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] RE: [Histonet] RE: Formic acid disposal?
The language is Korean. The message or
I have recently moved to a new organization and am setting up a new surgical
pathology lab. The lab LIS system is Cyberlab and I am told that they have a AP
module. In the past I have worked with Cerner Classic and CoPath Plus at a
hospital but this lab will be a much smaller set-up. Obviousl
OK Jay, I'm going to have to make this drink of your - thanks for the recipe.
How thick were these whole rabbit mounted sections? Were the giant glass
slides gelatin subbed?
I was thinking of mounting a couple of these large 100um brain sections & see
how good penetration is for the antibodies. I
Dear Histonetters,
Does anyone have an algorithm for cost per block on the Sakura Xpress? I would
need it for biopsy specimens only. I inherited one of these machines and before
I put it into use, I want to know what it will cost me and yes I have reached
out to Sakura (Florida) but not all my q
Dear Histonetters,
Does anyone have an algorithm for cost per block on the Sakura Xpress? I would
need it for biopsy specimens only. I inherited one of these machines and before
I put it into use, I want to know what it will cost me and yes I have reached
out to Sakura (Florida) but no responses
Wish you a Prosperous and Exciting.
2015
We look foward to working with you this year!!
NEW ARRIVAL:
[1]BD FACSCanto II With Fluidics Cart
Manufactured in 2008
Dual Laser, 7 color set up
D
NEW ARRIVAL:
[2]Biacore T-200
- Upgraded from a Biacore T-100
[3]ABI 3500- 8 Capillaries
NEW,
Does anyone know if the VIP 6 cassette baskets fit other vendor embedding
centers? Spec sheets have a lot of measurements but practical experience is
the best guide.
We are considering a Thermofisher embedding center or a Leica Embedding Center
but most likely we will process with a VIP 6 so
Hello Patsy,
Thank you very much for responding! Yes & of course, I'll be removing the
celloidin from each section - we normally
do this on smaller human whole brainstem sections using ether/100% EA 1:1 - 3x
- 3 minutes each with agitation. The
latter type of sections are very easy to work wit
Greetings,
I am looking for fixatives that others are using in their labs
for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in
10% NBF. Thanks in advance for any info on this.
Tiffany
DISCLAIMER:
This message is intended for the sole use of the addresse
PATH MD, a state-of-the-art reference pathology laboratory located in Los
Angeles, CA, is looking for two full time histotechnicians to start sometime in
the first quarter of 2015. Applicant must be a motivated team player and have
at least one year of experience and be ASCP registered. Profi
Are any of you making your own HER2 IHC control slides from patient tissue?
~~
This email and any files transmitted with it are confidential, and intended
solely for the use of the individual or entity to whom they are addressed.
for the whole bee I probably would process and embed it in glycol methacrylate
(gma) it is much harder and would give better sections, we have done zebra fish
and several other harder tissues including calcified bone in GMA.
Cheers,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E A
Tiffany,
Have used 10%NBF on muscles but also alcoholic fixatives -alcoholic formalin or
absolute- just always preferred 10%NBF since it gave the morphology and
counterstaining I wanted. diastase in a 6.0pH buffer (don't heat above 40
degrees if trying to speed up heating-kill the diastase) an
The oddest things I cut were the honey bee and yellow jacket stingers. I've
done plant stamen, reptiles, fish and I believe another insect. I usually tell
the students that are working on a research project to give me a sample they
don't care about so I can see if I can do what they want.
But
I have seriously Important Info how to keep your costs as low as possible. We
were the first lab to implement this plan and it works perfectly for us! Give
me a call for details.
Jeanine Sanders
CDC Atlanta
404-639-3590
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hello colleagues,
I would appreciate any recommendations for an automatic slide stainer. It
will primarily be used for H & E staining, not IHC.
Thanks in advance,
Carla
Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 U
Yes, 3 different antibody expressions per block.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-b
Hello Histologists:
Is there anywhere to get this ? Celestin Blue B C.I. 51050
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I don't know if the slides were subbed or not, but they looked
improvised. The point is, at some point the sections are going to have to
be mounted for visualisation, right? Someone or something is going to look
at the section under magnification?
If you mount them on glass *before* st
The coolest thing I cut was 600 yo deer bone. One of our pathologist did
archeology as a hobby and wanted me to section it, it was petrified as hard as
a rock. We tried everything to soften it to no avail. We ended up cutting
with a diamond wire lapidary saw without embedding it in anything.
When I worked in a research core (which was only last week, but I just changed
jobs). I have processed and cut (with varying degrees of success) fake meat
samples for a company. They were mainly made of grains and mushed veggies.
The problem was the samples were not consistent and there was no
I processed honeybees and was successful sectioning them. It takes a bit of
patience and time. I soaked the bees to soften the outer parts in glycerin
water and or mollifex. As for processing, I had to make up a schedule and
infiltration is the key. If it isn't important to see the whole bee you
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