[Histonet] p16 Antibody

Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! Véronique __

[Histonet] Texas Society for Histotechnology 2015 Sympsoium Convention March 20-22, 2015

Hi Histonetters! The Texas Society for Histotechnology will be holding the 2015 S/C in Plano Texas. If you are interested in a electronic version of the program please send me a e-mail via this communication. We would love to have you join us this year! Regards, Kathy Dwyer Texas Socie

RE: [Histonet] p16 Antibody

We use one from BD, Veronique, with good results. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] p16 Antibody

Ventana unfortunately has the market on the "good" clone...you can dilute it out pretty far (it comes pre-diluted) and still get good results however... Sarah Dysart BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor North Austin Medical Center 12221 North Mopac Expressway Austin, TX 78758 512-90

[Histonet] MYC-N antibody

Good Morning, I am wondering if anyone in the clinical world is using MYC-N antibody? I would like to know who the manufacturer is and what your protocol may be. I will be running these in a research environment, first on cells and then potentially on research tissue. Your help will be greatly

RE: [Histonet] p16 Antibody

We use the Ventana (INK4a) clone as well. What dilution do you use? Brandy Burnett Histotechnoligist, QIHC(ASCP) CCH Pathology/Histology 508-862-5267 bburn...@capecodhealth.org Expert physicians. Quality hospitals. Superior care. -Original Message- From: histonet-boun...@lists

RE: [Histonet] p16 Antibody

We dilute ours 1:5 from the predilute and run it on the Bond 3. It works very well.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message-

[Histonet] breast tissue and radioactive seeds

Hi Everyone, After 5 years of being in the private sector I am now back in the wonderful environment of a hospital setting as the Histology supervisor. We are going to be starting a new procedure here called "Conversion to Radioactive Seed Localized Breast Surgery" and I have been asked to come

[Histonet] RE: breast tissue and radioactive seeds

If this is the I-125 seed localization procedure for breast casses: our Radiology dept here does this and I can tell you what we do here. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -

[Histonet] Tissue exchange- Coccidiodes for fungus

I checked with NSH and they say that their tissue exchange bank has been discontinued. I have a lot of lung tissue containing Coccidiodes organisms (Valley Fever). I would like to trade for some tissue containing fungus. Is there another tissue exchange network out there? Is anyone intereste

[Histonet] RE: Tissue exchange- Coccidiodes for fungus

Hi Jeff, The Michigan Society of Histotechnologist has a tissue exchange bank available to all MSH members, so you might want to check with them. Regards, Vincent Rivera, HT (ASCP) QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vriv...@westderm.com -Original Mes

RE: [Histonet] Cassette lableler and slide labeler

James, I think I can respond to this as I am an LIS vendor. The short answer is that it should NOT be an issue, ASSUMING your LIS vendor can communicate/interface with the two instruments. Once the barcode is on the cassette or the slide, it becomes a standalone entity. The barcode on the casset

[Histonet] RE: Cassette lableler and slide labeler

Jim, by "system" do you mean using General Data software/printer to produce the barcodes, outside of your LIS, or just using the cassette printer with your LIS? If you are using a third party system to track materials outside of the LIS, then everything downstream has to use that system as well,

[Histonet] MMA Troubleshooting for Undecalcified Bone

This is intended for Bone Histology samples embedded in MMA. I had two main questions. 1. After mounting my tissue sections (5-10microns) with Haupts adhesive, I noticed that the bone within the section was appearing opaque/darkened on the microscope? I will add that the morphology of th

[Histonet] marking small samples

Dear Histonetters, Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? Preferably something that would not interfere with subsequent antibody staining. Yves

RE: [Histonet] marking small samples

Eosin in the alcohol works great to mark these small pieces of tissue. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com www.clinlab.com -Original message

[Histonet] New Histology Opportunities - CA

Hello Histonet! We hope everyone is having a great 2015. One of our leading clients has opened a few Field Based Histology Support opportunities based on the west coast. The ideal candidate will have an IHC background and interested in a customer facing support/specialist type opportunity.

RE: [Histonet] marking small samples

We use eosin the processor alcohol. Also we use blue-tinted paraffin which helps with contrast on small bx Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, includi

[Histonet] Message to renew subscription.

Got a message to renew subscription or it will be cut off.? Would like to continue receiving histonet.? Is this message from some criminal or from histonet?? Alice Neumann MD Pinnacle Pathology PC 207 Auburn Dr. ?#1 Auburn, AL 36830 24 Hour Ce

Re: [Histonet] marking small samples

I've been told Eosin affects a lot of molecular testing. We used to use it in our process but discontinued to not interfere with future testing. Sent from my iPhone > On Jan 14, 2015, at 5:31 PM, "Morken, Timothy" > wrote: > > We use eosin the processor alcohol. Also we use blue-tinted para

RE: [Histonet] marking small samples

Eosin can be a problem in IHC as well especially if you use alk phos red detection. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com > From: mbu...@atlanticurologyclinics.com > To: timothy.mor...@ucsf.edu >

RE: [Histonet] marking small samples

Last year I found a paper to the effect that eosin may interfere with PCR analysis. Our molecular group did an internal study and did not find any significant issues. But I just found another paper stating no interference: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3383644/ Am J Clin Pathol.

RE: [Histonet] marking small samples

Thanks for your research Tim, this is good to know. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com > From: timothy.mor...@ucsf.edu > To: mbu...@atlanticurologyclinics.com > Date: Wed, 14 Jan 2015 23:28:49

[Histonet] Of possible interest to some Histonet Listers

Dear Histonet Listers: The following link, http://blaypublishers.com/category/previous-issues/ , contains the hitherto published issues of a peer-reviewed, scientific journal I run, called* Life: The Excitement of Biology*. Some of the articles deal with subjects of possible interest to