I am working on setting up an adequate validation study for tissue processing
at a new lab. I want it to be adequate but also not too labor intensive or
costly. I have been in Histotechnology for over 36 years and have done this
before in the lab I can from. Unfortunately sometimes I make
We also use the little table-top band saw from Mar-Med. It has a small
footprint, splash guards, and is just awesome for cutting thin beautiful
sections. We use toothed forceps to hold the specimen and keep fingers
out of the way. Like the man said, a hot knife through soft butter! We
have 3
Hi Jim,
While I do not have quite the length of time in the field that you do, I do
have been through this several times and have some thoughts.
First, to your question about how many samples is acceptable, I would say
you should somewhat base this on your acceptability criteria. For example,
if
What is clean-up like?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Wednesday, February 18, 2015 12:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Bone Saw
We
In the event you don't have a band saw, I wish I did, we have a vice that
has a suction cup bottom that we attach to the top of one of our specimen
carts. It holds the bone well enough to get reasonable sections. If I had
to do too many more than I do, I'd request a band saw. When I'm done, I
Hi all,
I need some help in locating an article for class, preferably one I don't have
to pay for. The name of the article is The Effectiveness of Honey as a
Substitute for Formalin in the Histological Fixation of Tissue.
The only place I found this article so far charges $48 dollars for
Several questions:
1. Like Pamela has asked - what's the processing schedule and what
regents are used? I've had some problems if the brain is soaked in EtOH too
long (70% or higher), it will cause chattering.
2. Do you slice the whole brain including cerebellum and spinal cord
Thanks to all who responded to my requested for information concerning
the release of tissue to patients. I seems that most facilities are not
releasing tissue, especially if it has been fixed in formalin.
Thanks,
Gary Martin
El Dorado Pathology Med. Grp.
Marshall Med. Ctr. Pathology Dept.
We have a position open for Histology Surpervisor at Providence Hospital in
Mobile, Alabama. Online application www.providencehospital.org.
Anita Dudley
Providence Hospital
Mobile, al
___
Histonet mailing
You can read it here:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768083/
Geoff
On 2/18/2015 2:48 PM, Fawn Bomar wrote:
Hi all,
I need some help in locating an article for class, preferably one I don't have to pay
for. The name of the article is The Effectiveness of Honey as a
If you are a member of NSH you can also get a pdf for free by way of nsh.org.
See email copied below.
John Kiernan
London, Canada
= = =
Dear All,
I am pleased to confirm that the login button for the Journal of
Histotechnology Editorial Manager site is now working.
You may need to
I sent her the .pdf file I downloaded from Many Pub.
Brett
Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803
-Original Message-
From:
I would be curious to know if there was formalin on the processor. They
only mentioned processing in a conventional manner.
Just curious!
Douglas A. Porter, HT (ASCP)
Pathologist Assistant
IT Coordinator
Sparrow / CAP-Lab, PLC
2508 South Cedar Street
Lansing, MI
Hi Kimberly,
Most of what I work with is mouse tissue and I've found the brains to be a bit
tricky because they both hydrate and dry out quickly. I normally keep the
paraffin blocks on an ice for about 2 hours (after they've been fully faced
in), checking periodically to make sure the tissue
How are you billing for bone marrow bxs. we do irons on smear and core plus
clot. should we bill for all iron stains? also we do a wrights stain.
Anita Dudley
Providence Hospital
Mobile, Al
___
Histonet
If you are a member of NSH you can get a pdf for free just by contacting the
staff at the NSH office. A great reason to join, if you have not already!
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA
My error, I sent a link to a similar article. The original is in The
Journal of Histotechnology. 2006;29(3):173–176 but my library does not
have that journal. Perhaps someone on the list has it.
Geoff
On 2/18/2015 2:48 PM, Fawn Bomar wrote:
Hi all,
I need some help in locating an article
I'll scan a copy I have and email it to you later today.
Jim
___
James E. Staruk HT(ASCP)
www.masshistology.com
www.nehorselabs.com
www.parascreen.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Being old school I was taught that antibodies do not get stored in a frostless
freezer. I am now being told that the new frostless freezers do not go above
-13°C during the defrost cycle and it is safe to use. Teach this old dog a new
trick, is anyone using a frostless freezer for storing
?Hello Histo folks
I am starting a research project with mouse brain, I am having trouble with
chatter on the regular 3.5 mm sections and cant get the 9-10 mm to cut at all.
I have soaked them in warm water and wonder in using a softener like
conditioner would help. I am new to the
Please give your processing schedule with times and reagents? It is hard to
help without knowing how the tissue was handled from sacrifice to embedding.
Pam Marcum
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
This article about using honey as a fixative
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768083/
contends that you can use honey, jaggery sugar (look that up in Wikipedia),
or sugar syrup as a fixative.
What you're doing with honey is creating a transport medium - like the
chemically more
Sorry but my itchy delete finger got rid of the subscriber who was asking
about this.
There is a 2014 article by Sabarinath et al in Journal of Histotech. vol 37
pp 21-25. comparing honey and formalin fixation.
If you use honey versus formalin as fixative and go to the go to
www.maneyonline.com
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