Schiff into 10 mL of 37-40% formaldehyde
From: Bernice Frederick b-freder...@northwestern.edu
To: Histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Date: 04/13/2015 12:40 PM
Subject:[Histonet] Schiffs
Sent by:histonet-boun...@lists.utsouthwestern.edu
Having a brain fart all- does the Schiff go into the formaldehyde or vice versa
to test if the Schiffs is still good?
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Does anyone have a reference for the theory of:
1. The use of soapy water to prevent fatty tissue from blowing
apart in the flotation bath?
2. The use of ammonia water to rehydrate tissues?
Many of us use these tricks, but is there a source for the theory?
Thanks,
Jennifer
Do you need a source to satisfy ignorant officials?
Try a good textbook that shows up in second-hand bookshops.
Brown,GC 1978 An Introduction to Histotechnology. New York:
Appleton-Century-Crofts.
It doesn't seem to have an ISBN.
John Kiernan
= = =
On 13/04/15, Jennifer MacDonald
Thanks!
From: Shannon Kleiner [mailto:shk...@bellin.org]
Sent: Wednesday, March 04, 2015 4:12 PM
To: histonet@lists.utsouthwestern.edu; Piche, Jessica
Subject: Re: [Histonet] p16
Hello Jessica,
We use Cintec p16 on our Bond Max. Here is a link you may find useful
Hello Florida Histonetters and Beyond!!!
I wanted to let you know that we only have a week and half left for guaranteed
room rate and availability for the hotel where our meeting is being held.
Please act quickly if you are considering attending our meeting. I am including
the content of a
Hello Histonet!
Thank you all for your time and wonderful suggestions. This is the very
first time, I have to work with paraffin embedded brain tissues.
Based on the suggestions from Histonet colleagues, I have decided to
obtain 200 um thick brain sections for processing and embedding. Since I
I used 88% formic acid in Dwater to pretreatment of mouse tissue slides
before beta-Amyloid and Anti-Glial Fibrillary Acidic Protein (GFAP)
antibodies IHC. 10 minutes in formic acid with gental shaking before
blocking step.
You may also check this paper.
Acta Neuropathol.
Last week I asked for suggestion to help out a young man at a University where
he had few resources to help him with a bone project. I had given him several
suggestions and wanted to be sure he got as much information as possible to
help him complete the project. Since it was not at a medical
