Hi
1) Our college is trying to save on reagents. Most textbooks say that
Verhoeff's working solution is stable only for a few hours. Someone told me
that he used to keep the same solution for up to a month. Has any of you tried
to reuse Verhoeff's working solution? If so, how long did you keep
Here is the CAP checklist requirement:
ANP.21450
All histochemical stains are of adequate quality, and daily controls are
demonstrated on each day of use for the tissue components or organism for
which they were designed.
Ray...you should call the CAP and ask for guidance on this.
My interpretati
Rachel
We also run multiple antibodies under multiple parameters and there is no need
or reason to cut in one ribbon that many sections. You will still be able
collect somewhat serial or the sections that you need with multiple ribbons.
You are only able to fit on the waterbath in one ribbon
*Arizona Society for Histotechnology Spring Quarterly Meeting*
*Saturday, May 9th*
Sonora Quest Laboratories/Laboratory Sciences of Arizona
1255 W. Washington St. | Tempe, AZ 85281
8:00-11:30
*Anatomic Pathology and Quality Process Improvement*
William DeSalvo
Lunch and Social 11:30-1:00
Hi
The long ribbons are for R&D and QA purposes. Often we test under multiple
conditions 1-4 with multiple antibodies to the same tissue or multiple
tissue to optimize reagents. It quickly adds up.
Rachel
On Mon, Apr 20, 2015 at 3:28 PM, Joelle Weaver
wrote:
> Why are you cutting such a long
The one question we have not asked is; are you cutting serial sections for
these blocks? If you are are you laying them out on a board and not a
waterbath? I have done that with neuropath sections in research for whole rat
and monkey brains. Then your ribbon length would make sense. Generall
No ice forms in fixed, paraffin embedded tissue blocks at usual temperatures
and length of chilling time and temperatures.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> Date: Mon, 20 Apr 2015 19:06:17 +0200
> From: j.benavi...@eae.csic.es
> To: histonet@lists.utsouthwestern.edu
> Subjec
Why are you cutting such a long ribbon? You usually only a need a series of 3-4
sections even for ribbon cutting. Might be easier to control if you don't try
to move such a long ribbon to the waterbath. Drag the shorter ribbon towards
you on the waterbath. Make sure the water is not too cool. Fa
If you process properly, no over dehydration, there is no reason to soak.
Keeping block cool will help in cutting. Sharp, clean blade a must. Correct
water bath temp and time are the most critical factors, after cutting, to
produce a wrinkle free section.
Sent from my iPhone
> On Apr 20, 2015
Hi there,
I´m curious about the soaking thing. We have never done it in our lab.
Which is the purpose to do it?
Than, after facing the blocks, we chill them in a cold plate so, if
wanting to do the soaking , when should we? I guess before placing them
on the cold plate, but that may cause a
Check you blade angle. Keep your blocks cold, change your blade often and just
be patient.
Tom Podawiltz HT (ASCP)
AP Section Head
LRGHealthcare
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rac
Rachel,
First off, are you chilling and soaking the blocks after you face them?
Do that and see if there is a difference.
Don't try to get many sections to your ribbons. Shoot for a smaller ribbon
(5-6) sections that are good. Cut slowly but consistently.
What microtome are you using? Are you usin
How thick are you cutting? Is the block cold? Are you using disposable
knives? Are you moving the knife when you start sectioning? How fast are you
attempting to cut?
What kind of tissue are you cutting? Do you know which paraffin is being used?
There are many reasons for wrinkles and thes
First make sure your block is cold. Second, some models of microtome's and
blades don't cut well without dulling the blade a bit.(newer models don't seem
to need it.) Lastly some processors don’t fix as well as others you may need
to face the blocks in and soak them. Again some places soak in
Hi
Thursday was the first time I ever used a microtome I move to a lab
that does not have someone dedicated to cutting. I already miss her.
I have no problems getting ribbons of 10-30 sections long but the pieces
are half the size of the original block. I am guessing they are wrinkling.
What
The95 for HIER is in liquid, The 82in the oven is dry slides. While wet high
temps enhance HIER, It seems the high dry temp does harm epitopes (there is a
paper somewhere on this but I don't have access to it right now).
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular S
We have used this set up for several years now for CT biopsies. We like the
setup because it is portable ( it is contained in a carrier for transport).
The reagents are good for about 3-4 uses (we use it rapidly so contamination
occurs quickly) and then it must be changed. The stains are chan
Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are
removing water trapped between slide and paraffin section. Antigen retrieval is
an entirely different process. So not try to combine the two processes
Sent from my iPhone
> On Apr 20, 2015, at 8:48 AM, Preiszner, Joha
Hi Netters,
is there something wrong with this logic:
"If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven."
Of course I'll test it before I try it on real specimens, but maybe someone
else already knows the answer...
Thanks!
Hanna Preiszner
ETSU/QCOM
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