[Histonet] Verhoeff's hematoxylin

2015-04-21 Thread André K
Hi 1) Our college is trying to save on reagents. Most textbooks say that Verhoeff's working solution is stable only for a few hours. Someone told me that he used to keep the same solution for up to a month. Has any of you tried to reuse Verhoeff's working solution? If so, how long did you keep

RE: [Histonet] RE: Nuclear Artifact

2015-04-21 Thread Joelle Weaver
When I had this occur recently and sporadically, it was a collection issue. Joelle Weaver MAOM, HTL (ASCP) QIHC From: lbla...@digestivespecialists.com To: ro...@labcorp.com; histonet@lists.utsouthwestern.edu Date: Tue, 21 Apr 2015 13:48:07 -0400 CC: Subject: [Histonet] RE:

[Histonet] RE: (no subject)

2015-04-21 Thread Mayer,Toysha N
-I think you can use the other tissue controls. Even CDC (Hansen's Disease Center) will send out mycobacterium leprae controls free of charge, and I think they are armadillo. The best control I have ever used for mast cells is canine mast cell tumors. I request them from Vet Schools

[Histonet] RE: Nuclear Artifact

2015-04-21 Thread Podawiltz, Thomas
I had this issue a couple of years back. Found out that there was a delay of over an hour from the time the specimen was collected to the time it was being put into fixative. Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -Original Message- From:

[Histonet] Nuclear Artifact

2015-04-21 Thread Roy, Lisa
Hi HistoNetters: I have run into quite a problem. My lab currently processes all tissue types from 3 different sites. Recently, we have been getting complaints from one of the sites that the biopsies have a nuclear artifact. It is described as washed out or poor to no nuclear detail.

[Histonet] RE: Nuclear Artifact

2015-04-21 Thread Blazek, Linda
The first place I would look is to what may be happening before they reach me. If it's only one site with an issue, it sounds more like an issue at collection. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

Re: [Histonet] (no subject)

2015-04-21 Thread Shruti Shah
Hi does any one doing mice tibia histology, we use to fix in formalin for 24 hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 24 hours long protocol in automatic processor. But I am facing problem with bone marrow shrinkage. If any one have idea for

[Histonet] Visual alert for processors

2015-04-21 Thread Bitting, Angela K.
Does anyone know of a way to generate a visual alert (hearing impaired device?) to notify us when our processors finish? They are going to be elsewhere in the building and we need to know when they finish. Baby monitor is out of the question due to privacy issues. Sent from my iPhone

Re: [Histonet] controls to lengthy off topic

2015-04-21 Thread koellingr
Hello Garrey, Curious myself, CAP contact info seems to be greyed out on website unless I officially log in and for now my concerns are with the Washington State Science and Engineering Fair for K-12 and golf game.   (1) There are at least two phrases in the ANP.21450 which could be parsed

[Histonet] Florida Society for Histotechnology Meeting

2015-04-21 Thread John Shelley
Good Morning Histonetters! Did you know or may be even care to know that we have only 3 days left to guarantee a hotel room for our 2015 Spring FSH Meeting. Final reservations should be made no later than April 23rd, 2015 to ensure rate and availability. After that date you will have to hope

[Histonet] IHC technician needed in Modesto, CA.

2015-04-21 Thread Taylor Rinaldi
A growing anatomic pathology laboratory in Modesto, CA is looking to hire a IHC technician for their Histology department. ASCP certification preferred. This is a permanent, full time opportunity offering relocation assistance. Please reach out to me for immediate consideration. Thanks so

[Histonet] IHC/SS QA/QC Sheets:

2015-04-21 Thread Jb
Can someone help guide me on the right direction regarding how to organize daily QC sheets. Currently we have one per case (this is time consuming and I'm on overload of papers). Does anyone have a good solution and are you willing to share? Thank you for your help, Craig Sent from my iPhone

[Histonet] Fwd: mouse tibia histology

2015-04-21 Thread Shruti Shah
Sent from my iPhone Begin forwarded message: From: Shruti Shah s.s...@garvan.org.aumailto:s.s...@garvan.org.au Date: 21 April 2015 7:28:07 pm AEST To: Garrey Faller garr...@gmail.commailto:garr...@gmail.com Cc: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu

RE: [Histonet] Verhoeff's hematoxylin

2015-04-21 Thread Tony Henwood (SCHN)
Suggested Answers below 1. several hours is all you will get out of the solution I am surprised there is a method that gives you a month shelf life unless it is a different method eg orcein 2. If it works keep using it 3. Allowing the alcoholic Hx to ripen for at least a few days will

[Histonet] autdialer for Peloris

2015-04-21 Thread Bitting, Angela K.
Can anyone recommend a third party auto dialer to connect to Peloris? Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by

Re: [Histonet] Nuclear Artifact

2015-04-21 Thread Bitting, Angela K.
We occasionally see this also. Sent from my iPhone On Apr 21, 2015, at 7:59 PM, Sue suetp...@comcast.net wrote: OMG we are experiencing the same issue. At first it was just GI and now we are seeing it on prostate. One pathologist said it looks like the tissue has been cooked. The only

Re: [Histonet] Nuclear Artifact

2015-04-21 Thread Sue
OMG we are experiencing the same issue. At first it was just GI and now we are seeing it on prostate. One pathologist said it looks like the tissue has been cooked. The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director

[Histonet] mice tibia histology

2015-04-21 Thread Dorothy Hu
Your decal time should be according to your mice age. Four weeks old mouse normally need four changes of EDTA in two weeks time. I think your process time is a bit too long. If your four weeks old mouse tibia, 13 hours from 70% ethanol to last infiltration paraffin should be fine. Your shrinkage

[Histonet] RE: (no subject)

2015-04-21 Thread Cynthia Robinson
A few years ago we got cited for not having the fungal control be in tissue. The citation was from a HQIP survey we participated in from CAP. We were using a cultured fungal specimen in a cell button at that time. Since then I have collected and shared a number of cases we have seen with fungal

[Histonet] Fwd: FW: IQMH Symposium, Toronto, ON CANADA June 4/5, 2015

2015-04-21 Thread June Shin
*From:* June Shin *Sent:* April-21-15 11:53 AM *To:* 'histonet@lists.utsouthwestern.edu' *Subject:* IQMH Symposium, Toronto, ON CANADA June 4/5, 2015 *The 2015 IQMH Symposium* The demands of personalized medicine are upon us, and it is critical that patient tissue specimens are confidently