We wish to measure amount of DNA in grapevine bud shoot apical meristem
(small 1 by 1mm)-any advice or modern references as to fixation,
permeabilization, best stain (proabably propridium iodide).
Regards Peter Noyce.
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To dry them overnight before fixation is not a bit too risky? I mean,
wouldn´t the tissue degradate? I normaly fixed the section as soon as I
get it into the slide.
Just a thought. I am no expert in cryostate, just an occasional user.
Julio
Lewis, Patrick patrick.le...@seattlechildrens.org
Patrick,
We do a lot of frozen section IHC work. Years ago Gayle Callis turned me on to
fixing in cold acetone:ethanol (3:1) . We keep it at -20C and I fix for 10
min. on the bench then wash in PBS and proceed with the IHC. We do dry slides
for at least 30 min before fixing. This has worked
As I usually process gross pieces of bone -that need abouth thirty
hours decalcifying- I suffer the same nuissance. Also would thank any
hint.
Pablo Sanchez
Laurie Colbert lcolb...@pathmdlabs.com escribiu:
I have tissue that was left in decal over the weekend and now has
very poor
What type of tissue cassette is being used? What type of insert or wrap is
used. If one cassette processes correctly and the next to it does not, hard to
say tissue processor is causing issue.
Sounds like a water problem and it could be water trapped in cassette. Check
the rest of you process
I would suggest not allowing decalcification to be extended. Better to leave in
fixative until you can control the time of endpoint of decalcification. What
type of decal solution are you using? I am not a big fan of trying to adjust
the chemistry of the stain to compensate for over
Good article on IHC world about restoring nuclear detail to over decalcified
tissueit is called Problem Number 23. Good luck.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent:
We are having similar issues with our tissue.
Any troubleshooting insight would be greatly appreciated!
Thanks!
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Sue
[suetp...@comcast.net]
Sent:
In the past I have plenty of years of experience recycling formalin and
clearing agents, however I don't have much experience with recycling reagent
grade alcohols. If we recycle waste alcohol that is 95% - 100 %, I am told
that our recycled product will be 99% or above. With that said how
In the past when using giemsa stain,
I came across two human cases of very long helicobacter organisms.. I was
stumped the first time since I had never seen one previously. I reflexed both
to immuno and both were positive with the h pylori antibody. I assume they were
both heilmani. I think it
Hello Everyone
It's that time of the year again. As you know this year's NSH Convention is
being held in Washington DC. The Stars Stripes NSH Awards Ceremony
Celebration will be on Saturday August 28th.
The NSH offers many awards, too numerous to list here, so to see the list of
awards
Jim, yes, in my experience you are going to use this for 70 to 95% alcohol
steps, not 100, unless you get a water absorber (BR has one, at least used
to).
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San
I prefer (and my pathologist) the Warthin Starry. I performed that routinely,
and affordably, on the Dako Artisan at my last employer.
At my current small lab we perform a Giemsa for h.pylori. I've seen two cases
of H. heilmanii in the last 2 years. Both stained with Giemsa.
When my lab
Helicobacter heilmannii (sorry I misspelled it before) was named
Gastrospirillum hominis when it was first described. Tight cylindrical
spirals, unlike the gull-wing (like you learned to draw birds in the sky
when you were in the fourth grade) morphology of H. pylori. I'm not sure
you could see
Nancy Stedman observes:
I believe IHC is more sensitive than the special stains too. One caveat
for anyone who works with veterinary samples - the H. pylori antibodies are
specific for H. pylori, so I have not found these antibodies to be helpful
for evaluating other species with
Has anyone had experience with the Leavitt Medical group and their BxChip
system Looking for ANY feedback so I can prove a point :)
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Hi,
If you are looking at the possibility of doing IHC manually, I would
really recommend the Shandon Sequenza. It is a box that holds clips that
attach to your slides. The slides are held vertically and you drop reagents
into the top. The reagents replace the previous reagent which drips
Good afternoon Histonet,
We got a complaint from one of our researchers this morning about loss of
antigenicity on an FFPE sample. According to the researcher the pERK antigen
in tissue is labile if not fixed right away, hence trying to find if the
samples which are negative are truly negative
I have tissue that was left in decal over the weekend and now has very poor
nuclear staining. Is there a fix for this so that I can get better nuclear
staining (other than restaining for a long time in the hematoxylin)?
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Part-time histotech needed for an independent dermatopathology lab in
Milwaukee, WI. This an approximately half-time position, though the hours
are somewhat flexible and negotiable. Good working environment and highly
competitive compensation. If interested, please contact the lab director by
Laurie and Pablo, doing a soft antigen retrieval in a citrate buffer
solution pH6 for 10 minutes after hydrating your slides
will allow you to improve the quality of nuclear stain.
El 29/04/2015 a las 13:07, pablo.sanc...@usc.es escribió:
As I usually process gross pieces of bone -that need
Thank you so much you to the ones who already replied to me! However I did not
receive any comment about the H1850 or H2250 from Energy Beam Science (EBS).
Does anyone have experience with any of these?
Thanks again in advance for a most appreciated prompt reply!
Liette Tougas, RT, B.Sc.,
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