The statement quoted by Tony from the Dako manual cannot be true because many
antigens have to be exposed to water at 100C in order to be immunostained -
antigen retrieval. Denaturation of a macromolecule by heat increases the number
of exposed epitopes, which typically are short amino acid sequ
I have been following this with interest both now and in the past.
A word of caution about the acetone/ethanol fixation. I did NOT use the
acetone/alcohol fixative cold, but at RT (as it was taught to me by an IHC
expert). That is a bonus since you don't have to maintain A/A fixative in
a
We do our billing manually through CoPath as well.
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Mike Pence
[mpe...@grhs.net]
Sent: Thursday, April 30, 2015 5:44 PM
To: 'WILLIAM DESALVO'; Cartun, R
Your Lis should not have done that.
If you are using Copath/Cerner, I think they have automated it now according to
their most recent newsletter. Currently, I have to manually change the 88342's
to 1's It's somewhat of a pain. But, your Lis should have consulted someone
before deleting all codes
We do all of our IHC billing manually.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Thursday, April 30, 2015 4:43 PM
To: Cartun, Richard
Cc: histonet@lists.utsouthwestern.edu
Subjec
Ouch!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Thursday, April 30, 2015 4:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC billing question
Effective January
We have to manually review the IHC billing also and continue to audit. It took
billing and IT three months to create the logic to automate billing for a
specimen and account for combination of there being the possibility of 88341,
88342 & 88344 and the proper combinations.
Sent from my iPhone
Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for
IHC from our CoPath stain dictionary since you couldn't tell whether a
Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you
might have expected, none of the "inpatient" IHC testing has been
This may sound like a really sophomoric question but I'll ask anyway. How do
you dispose of specimen containers from your histo tissues once they are
emptied and rinsed? This question came up at work (veterinary diagnostic lab)
and I am not aware of a standard or a reference to go to for an ans
Usually I have done weekly , but in rather high usage places. With more strict
downtimes and decontamination for TB or other suspected infectious cases.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: dknut...@primecare.org
> To: histonet@lists.utsouthwestern.edu
> Date: Thu, 30 Apr
Make your own with NaOH.
Dissolve 40 g NaOH in about 90 ml of distilled water. Use caution, the
reaction is exothermic.
When dissolved dilute to 100 ml with distilled. Keep tightly closed to
avoid picking up CO2 from the air.
Geoff
On 4/30/2015 3:35 PM, Bernice Frederick wrote:
All,
I have a
Bernice, You have to buy the 10N solution. You can only dilute a given normal
solution, you cannot concentrate them.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Orig
What about HIER at 95-98ºC? "Everybody" uses it to "enhance" epitopes
detection.Is there not an intrinsic contradiction here?
René J.
On Thursday, April 30, 2015 3:32 PM, Tony Henwood (SCHN)
wrote:
Yes,
I read the Dako IPX educational guides (5th ed) and on page 32:
"No processe
All,
I have a procedure here that call for and I quote "1.25 ml NaOH 10N in 1L of
water." I know how to get 1 N, but how do I get 10. Having rarely hd the
opportunity to make many Normal solutions ,my brain is not computing. Is it an
error?
Bernice
Bernice Frederick HTL (ASCP)
Senior Research T
Yes,
I read the Dako IPX educational guides (5th ed) and on page 32:
"No processes should raise tissue temperature to higher than 60oC as this will
cause severe loss of antigenicity that may not be recoverable"
Unfortunately there is no evidence given or cited that validates this
statement. Eve
Hi fellow Histonetters -
I was wondering if I could get some feedback from my peers on how you are
dealing with the CAP standard
ANP.23410 on Cryostat Decontamination.
It states that this is done at defined intervals appropriate for the
institution.
The place I just inspected was doing a weekly
Hi Everyone,
can some one give me advice am I able to use metal staining rack for TRAP
stain. Does Pararosaniline Dye or nepthol not going to react with metal or
create contaminant.
Thank you in advance.
Shruti Shah | Research Assistant
Bone Biology Group
Bone Biology Division
Garvan I
Hello, We have the Pathology Devices Semi automated units. We are happy with
them. Our decision to keep with the less automated units are largely due to our
work flow. We make TMA's for customers. But the customer has to do all of the
work in the design and data entry into out TMAJ Database. I t
I was wondering how many people have semi-automated or automated tissue
microarray equipment? I am especially interested in those who graduated from
manual methods to the automated and how they reviewed and ranked the limited
options out there.
Thank you!
_Sally
Sally Ann Drew, MT(ASCP)
UWSMPH
How long do you bake slides for before staining, at what temperature? Does
your stainer use agitation?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carolyn Nelson
Sent: Thursday, April 30, 2015 11:41 A
Hi, I was hoping someone can help me with tissue falling off the slides. I have
tried regular slides with and without adhesive in the water bath. Charged
slides with and without adhesive in the water bath. I have not changed the type
of slides I’m using. All the chemicals are fresh in the proces
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