I'd like to know how many labs use an extended protocol to process fatty breast
tissue. Does an extended protocol really work. I still believe if the tissue is
submitted in small, thin and adequately fixed sections prior to processing
there should be no need for a 16 hour processing cycle.
I agree if the tissue is cut properly you should not need an extended program
but when you have residents and PA's they tend to rush and their sections are
thicker. We do not have an extended program still within the CAP limits and it
works quite nicely.
TJUH
Sue
That's what we do as well.
Martha Ward
Wake Forest Baptist Health
From: Weems, Joyce K. [joyce.we...@emoryhealthcare.org]
Sent: Friday, May 01, 2015 9:13 AM
To: 'Cartun, Richard'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC billing
All that matters here is the final concentration of the reagent - it doesn't
matter what stock you start with if you calculate the dilution.
Adding 1.25 ml to 1000 ml is diluted by a factor of .00125 so 10N x .00125 =
.0125 N final concentration.
If using a 1 N stock solution, just add 10X the
Huh?
Take a solution more dilute than you want it and dilute it more?
Geoff
On 5/1/2015 10:41 AM, Goins, Tresa wrote:
All that matters here is the final concentration of the reagent - it doesn't
matter what stock you start with if you calculate the dilution.
Adding 1.25 ml to 1000 ml is
We have 2 billing codes for IHC. One for the first IHC and another for all
additional IHC's on the same case.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct |
And what LIS to you have?
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's
Hospital and is
Meditech
Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org
-Original Message-
From: Weems, Joyce K.
The final concentration of 1.25 ml 10N NaOH into 1000 ml water is the same as:
12.5 ml 1N NaOH into 987.5 ml water.
-Original Message-
From: Geoff [mailto:mcaul...@rwjms.rutgers.edu]
Sent: Friday, May 01, 2015 9:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Question
Hello,
I think I already know the answer but I'm not sure why so if someone can
help me understand the theory behind it, I would greatly appreciate it.
Currently, we use the Richard Allen kit for the GMS stain and it uses
Periodic Acid as the 1st step.
We use a control tissue from a
Am I misunderstanding, or should it be per specimen and not per case?
Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369
-Original Message-
From: Horn, Hazel V [mailto:hor...@archildrens.org]
Sent: Friday, May 01, 2015 8:50 AM
To: 'Weems, Joyce K.';
Does anybody have an idea how to attach the combination bracket with a shelf
for the Printmate from Thermo Fisher. No instructions and trying to
conceptualize how this thing goes together. Worse than putting together a
swing set or new kid's bicycle. Diagram or picture with one attached
To get a positive PAS or GMS fungal stain, one must oxidize the carbohydrate in
the fungal cell wall.
Chromic acid is a stronger oxidizer than periodic acid, so would work better
with mature fungal cell walls that are highly polymerized.
Treat an immature cell wall for too long, and you may
We have CoPath.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's
Hospital and is intended for
Hi Paula,
The same site of infection for your control and patient tissues does not
mean the fungus species was the same. Now for addtional commentary.
The classic GMS uses chromic acid as the oxidizer, stronger than periodic
acid. This is something to beware of since you had a false
Hi Gayle -
We have never had a noticeable problem detecting fungi using 5% aqueous
chromic acid at room temperature for 1 hr. The presence of mature and immature
wall structures may be the reason. We have never tried to determine the end
point for over oxidation, but my best guess is that it
Does anyone have a procedure that they can share on tissue transfer? One
stained slide to another?
Thank you for your help.
Sincerely,
Craig
Sent from my iPhone
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Hi Rene,
Contradiction? Possibly but HIER is done in an aqueous environment, whereas the
oven heating above 60oC was done dry (I will send you a copy of the article
under a separate email).
Regards,
Tony (from down under, currently in London UK).
From:
Hi Charles,
In this study, having previously heated the tissue to 80 degrees would improve
and speed up antibody-antigen reaction by ensuring the antigen was denatured.
But not all antibodies, some were not affected, others were adversely affected.
The cell and cell molecules are complex and
Hi histonetter and specifically Gayle Callis,
I try to explain why we need to leave MMA embedded glass vials in water
bath during embedding to a research lab. But I need experts to have a
convencing explaination. The lab use vacuum desiccator (which has sealed
ring to keep vacuum) to leave the
I assume that the dako manual is referring to dry heating above 60oC, not HIER
which is done in an aqueous environment.
The paper I quoted (which I will send you) found that it depended on the Ag-Ab
combination that was being used, some antigens were irretrievable after dry
heating where as
Slide Repair
1. Soak coverslip off of slide in xylene.
2. Piece slide back together.
3. Apply Mount-Quick to slide, covering tissue. Allow to dry overnight.
4. Place slide in cold water in refrigerator overnight.
5. Carefully peal the Mount-Quick off the slide. The
With Mount Quick you can also decolorize the section and do a different stain
on them. I have even performed IHC on these transferred sections when needed.
Shirley Powell
-Original Message-
From: James Watson [mailto:jwat...@gnf.org]
Sent: Friday, May 01, 2015 4:21 PM
To: 'Jb';
I have used this for IHC, as well. It's great stuff! You can also use other,
very viscous mounting medias, but the time it takes to dry/soak are longer than
with Mount-Quick!
Thanks,
Bonnie
-Original Message-
From: Shirley A. Powell [mailto:powell...@mercer.edu]
Sent: Friday, May
It's per specimen - 88342 for the first ab on a specimen, 88341 for ea
additional on same specimen. (Not multiple blocks on same specimen).
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody
Interesting! Thanks!
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's
Hospital and is intended
Histologists enter all TC IHC billing codes manually as performed before they
leave the lab.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: garr...@gmail.com
Date: Thu, 30 Apr 2015 17:52:25 -0400
To: mpe...@grhs.net
Subject: Re: [Histonet] IHC billing question
CC:
Hi Tresa,
What staining parameters do you suggest for seeing mature and/or immature
fungal cell walls? I don't think you will know what level of maturity is
present before doing a fungus stain. But wouldn't both levels of maturity
both be present if the fungus is actively growing in a
We have a great workaround in built in CoPath.
Every antibody in our IHC dictionary, is built in triplicate.
One is called [Ab name]-Primary with a 88342 CPT code,
the second is called [Ab name]-Add with an 88341 CPT code.
The third is [Ab name]-NC for no charge or N/A in the CoPath
I totally agree and if I might add:
Pneumocystis jiroveci are difficult to see after PAS staining because of the
strong mucin background staining. GMS is also more advantageous because it
stains old and non-viable fungal elements more efficiently than PAS (Henwood,
A. F., Prasad, L., Bourke,
Hi Teresa,
Very interesting.
Do you have any references on mature and immature fungal cell walls and how
they react histochemically?
Regards
Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist, the Children’s Hospital at Westmead
Adjunct Fellow,
Hi,
I have used a product called Mount Quick. It is designed as a liquid
coverslip, but once applied, it can be removed lifting the sections along
with it. Here is a link with a good description of the process.
http://www.newcomersupply.com/product/mount-quick
Happy Friday,
Amos Brooks
On
I would strongly suggest that you not use Periodic acid for the GMS, its
alright for the PAS but for the GMS. Use chromic acid as described the standard
texts.
Regards
Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist, the Children’s Hospital at
I do it every day - change every first to 88342.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint
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