Re: [Histonet] Paraffin block disposal

2015-06-06 Thread Brendal Finlay
I agree with Brian, but we dispose of  blocks by treating them as regulated biohazard waste. We also blocks them longer than 2 years. The CLIA regulation states keeping them for a minimum of 2 years. Outside facilities frequently request unstained slides or blocks on cases that are more than 2

Re: [Histonet] tissue fixation-formaldehyde concentrations which is best.

2015-06-06 Thread Tony Henwood (SCHN)
John, I totally agree Tony From: John Kiernan [jkier...@uwo.ca] Sent: Saturday, 6 June 2015 2:31 PM To: Peter Noyce; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue fixation-formaldehyde concentrations which is best. Dear

Re: [Histonet] [EXTERNAL] Re: plants in the lab

2015-06-06 Thread Tony Henwood (SCHN)
I agree with Paula. The way you have explained the regs implies that even I would have problems entering the labs. Maybe ban people altogether and then we meet the regs. Lets keep it real and not fantasy. Tony From: Boyd, Debbie M [dkb...@chs.net] Sent:

Re: [Histonet] plants in the lab

2015-06-06 Thread Tony Henwood (SCHN)
Laboratory Air-conditioning systems should be separate from patient area systems (as is required for operating theatre units). I understand this to be good practice. From: Goins, Tresa [tgo...@mt.gov] Sent: Thursday, 4 June 2015 12:18 AM To: Tony Henwood

[Histonet] Pre-floating tissue sections in dilute alcohol

2015-06-06 Thread Garrey Faller
Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf Anyone out there float their sections in

Re: [Histonet] Pre-floating tissue sections in dilute alcohol

2015-06-06 Thread tjfinney2010
Yes I use it for brain. Happy Connecting. Sent from my Sprint Phone. -- Original message-- From: Garrey Faller Date: Sat, Jun 6, 2015 10:48 AM To: histonet@lists.utsouthwestern.edu; Subject:[Histonet] Pre-floating tissue sections in dilute alcohol Hi everyone, I just became aware

Re: [Histonet] Decalcification of bone marrows

2015-06-06 Thread Bob Richmond
Adrienne (where?) asks: I have a really quick question: about how long does it take to decal a bone marrow biopsy? to which Jessica (where? - apparently in the US though) replies It all depends on what you use for decal. We use 5% Nitric acid for 1 hour or so. Sometimes it needs a bit more time.

Re: [Histonet] Pre-floating tissue sections in dilute alcohol

2015-06-06 Thread Joana Moreira
I Garrey, I came across floating sections in dilute alcohol back in 2005 when I started working in the UK and have been using it ever since. I agree it introduces one more step and wouldn't use for every single section - there's no need. But I do find it very helpful with certain blocks/tissues

Re: [Histonet] tissue fixation-formaldehyde concentrations

2015-06-06 Thread Hobbs, Carl
I have to agree with the student, John. Sure, he is coming from ignorance ( not a bad situation: naivety is not a fault... we all are/were there at some point;-) Sure...I agree with you re the using of the word Paraformaldehyde as a fixative I often sigh when used. However, differential

Re: [Histonet] Paraffin block disposal

2015-06-06 Thread Aimee Tolentino
That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta rarba...@csdermatology.com wrote: Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? DISCLAIMER:

Re: [Histonet] Paraffin block disposal

2015-06-06 Thread Cooper, Brian
Hey Aimee, This has been discussed several times on Histonet. It sounds like it depends on the institution. Since they're FFPE, pathogens are not a concern. I didn't reply to all because someone will shout out, What about CJD? and then I would have to punch them. They should be able to go into