Hi Histonetters! We are looking for a full time licensed histotech here in our
very busy Delray Florida Dermatology Lab. This is a permanent full time SECOND
SHIFT (40 hours) position with benefits (medical/401k/vacation) and competitive
pay. THIS IS A DRUG FREE WORKPLACE. Drug testing,
Does anyone knows how to measure pH in tissue?
Any help will be greatly appreciated.
Thank you.
Lin.
Lin S. Bustamante, B.S., H.T.(ASCP)
VIBS Histology Laboratory Supervisor
College Of Veterinary Medicine
Texas AM University
College Station, Texas 77843-4458
Phone: (979) 845-3177
Fax: (979)
We are trying to create a consistent and efficient process for the triage of
Radiology specimens going to Cytology, Surgical Pathology and Microbiology. I
would really like to hear how other hospitals are handling this. Do you have a
central drop off location and if so who handles this? Does
Does anyone have any recommendations for antibodies and or is aware of specific
issues/limitations for Beta 2 adrenergic receptor IHC? Working with murine WT
KO, PFA fixed frozen sections. Either IF or chromogenic are options...Open
to any guidance or suggestions ?Thanks
Hi Histonetters! We are looking for a full time licensed histotech here in our
very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT
SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift
differential. THIS IS A DRUG FREE WORKPLACE. Background check,
Why shouldn't the slide surfaces remain positively charged after removing the
sections? Water, alcohol and xylene are all routinely used, and don't impair
electrostatic forces that help to hold the sections on the slide. The
positively charged surface is not a coating that can be washed or
Rachel,
We dewax off line. We use the same protocol that we do for any other staining;
3 min in 3 changes of xylene (we use a xylene substitute though), 3 min in 2
changes of 100% alcohol and one 3 min in 95%.
We use BioCare's IntelliPath immuno stainer. It is a completely open system.
Dear Colleague
I just want to remind you..
After all of that procedure of removing the paraffin sections from the
positive charged slides.
The slides will not be positive charged anymore and not suitable for IHC.
Also the cost of the detergents and the chemicals which you used for
removing the
Your 2 minutes would be better spent looking in an immunohistochemistry
textbook. A small but excellent one is Polak, J.M. and Van Noorden, S. (1997).
Introduction to Immunocytochemistry, 2nd ed. Royal Microscopical Society
Microscopy Handbooks, 37. Oxford: BIOS Scientific Publications.
You
Hi Anna,
You could deparaffinize/rehydrate to 10% (or so) bleach. Section(s) comes
right off after ~1-60 minutes (or so). Did this by accident (that was a bad
day). Deparaffinzation via Rene J Buesa's DWS
(http://www.histosearch.com/ADP7HistologyWithoutXylene.pdf) method, or simply
Ventana loves that excuse for stain failures
They even made us flip our slides upside down to place the control on the slide
then flip them around to place the section on the slide due to the
electrostatic forces
Additionally all slides had to be air dried then placed in slide boxes prior to
Hi,
I am conducting a short 2 min survey for my science/business class
examining current trends for antigen retrieval also known as heat induce
epitope retrieval. Response will be greatly appreciated!
https://www.surveymonkey.com/s/7989LKR
Best,
Craig Vollert
Graduate Student
Department of
12 matches
Mail list logo
