It isn't very clear, but for the Formic Acid/sodium Citrate buffer you have
to use trisodium citrate. 10 g of disodium or monosodium citrate wouldn't
work as well...
--
Tyrone Genade
Orange City, Iowa
tel: (+1) 712 230 4101
http://tgenade.freeshell.org
*
Does anyone perform EMA/MUC1 IHC on canine tissue? If so, what company is
your vendor? You can reply to me in a private message. Thanks in advance.
--
Jan Shivers
Senior Scientist
IHC/Histology Section Head
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333
Instead of an antibody, you might consider enzyme activity histochemistry,
which is much less expensive.
Demonstration of 5-nucleotidase activity in the presence of levamisole detects
lymphatic endothelium. Sections can also be stained for alkaline phosphatase
activity in the endothelium of blo