Re: [Histonet] paraffin sectioning possibly calcified heart valves

2015-08-13 Thread Karen Stephanson via Histonet
Have you tried using RDO on the block surface? On Aug 12, 2015 12:30 PM, Jennifer Connell via Histonet wrote: Hello, I am having trouble with sectioning of paraffin embedded human aortic valve samples. I received the embedded samples from a collaborator who had the embedding done by the patholo

Re: [Histonet] are you desperate to hire a BS.c graduate

2015-08-13 Thread Barry Rittman via Histonet
Jose brings up some good points. It seems to me that we often spend too much time talking about employing people who have a narrow field of expertise, even though such expertise is necessary to get the job done. I would suggest that we instead look at the bigger picture. Experience outside your c

Re: [Histonet] Xylene Free Labs - Coverslipping and Frozen Section Questions

2015-08-13 Thread Rene J Buesa via Histonet
1-After you oven dry your stained sections, you use the very same medium you always have used. I used Permount.2- Given the very special constrains of FS (ready for diagnoses within 20 minutes of receiving the specimen) I used an aqueous mounting medium. After the diagnosis was made, I eliminate

Re: [Histonet] are you desperate to hire a BS.c graduate

2015-08-13 Thread deGuzman, Jose R via Histonet
Please correct me if I'm wrong but this has been my experience so far. I came from a research Histology background where I learned many skills that I was able to developed in the clinical side. I spent 3 years preparing for the exam because my manager in research and a co-workers failed on their

[Histonet] Xylene Free Labs - Coverslipping and Frozen Section Questions

2015-08-13 Thread Brendal Finlay via Histonet
For those labs who are xylene free I have two questions:   1. What mounting medium do you use when coverslipping oven dried slides? 2. How are you running down and coverslipping frozen sections after staining them?   I'd like to make sure the stains are preserved over time and my experience with

[Histonet] test

2015-08-13 Thread Amy Lee via Histonet
Hello, I am just wondering if I myself will see the email I sent out to histonet. Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Opinion on PTlink from Dako

2015-08-13 Thread Julio Benavides via Histonet
Hi, we are thinking about incorporating the PTlink system, from Dako, in our lab. Have any of you worked with it before? Any opinion? They would be greatly appreciated Thanks a lot Cheers Julio University of León Spain ___ Histonet mailing list

[Histonet] Evaluation of microtome

2015-08-13 Thread Arun Jyothi S.P via Histonet
Dear Teachers I am asked to evaluate one new microtome installed in our lab. I will be very grateful if you can help me to make an evaluation report. What are the things to note and if you have any sample evaluation report please sent me so that I can make a template from that. Thank you Arun H