Re: [Histonet] IHC on old slides

2015-11-13 Thread Hobbs, Carl via Histonet
Amplification is the only way. Possibly, tyramide amplification will help. It will cost you to buy a kit...sure, make your own butyou have to weigh up pros/cons Sureyou will have to play with variables. ___ Histonet mailing list Histonet@lis

Re: [Histonet] #ExtMail# Slide and cassette printers

2015-11-13 Thread Ellenburg, Deborah via Histonet
We have the ShurMark slide and cassette engraver that interfaces with CoPath. Sent from my iPhone > On Nov 12, 2015, at 10:50 AM, Mike Pence via Histonet > wrote: > > Okay, I am reaching out to the people that I know that has an answer to > everything. What system are you using to print sli

[Histonet] Mohs sections

2015-11-13 Thread Angela Yepes via Histonet
> From: Angela Yepes > Date: November 12, 2015 at 6:05:57 PM EST > To: histonet@lists.utsouthwestern.edu > Subject: Mohs sections > > >> From: Angela Yepes >> Date: November 12, 2015 at 4:15:50 PM EST >> To: histonet-requ...@lists.utsouthwestern.edu >> Subject: Mohs sections >> Hello everyone

Re: [Histonet] IHC on old slides

2015-11-13 Thread Rene J Buesa via Histonet
The only thing you can do is to prolong the Ab staining time but, in order to avoid excessive background, dilute it. You will have to find an adequate balance between a more diluted Ab with a weaker spitope signal and a  prolonged incubation time and there is no "magic formula" for obtaining it.

[Histonet] IHC on old slides

2015-11-13 Thread Stoll, Kathryn via Histonet
Hi Everyone, I have been doing some IHC staining on slides that are 20 years old or more. Depending on the antibody the staining is pale in comparison to my control slide. Does anyone have experience with staining older slides? Do you have any tips to share? The slide storage conditions ar

Re: [Histonet] Histology Related Questions?

2015-11-13 Thread Rene J Buesa via Histonet
That is OK with me.René On Thursday, November 12, 2015 7:48 PM, Va Paula Sicurello via Histonet wrote: Hello Netters, I am taking a research techniques class for my MBA this term and need to have a project that I can ask quantifiable questions.  I was thinking about productivity a