Re: [Histonet] DIF on paraffin embedded tissue

No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is USELESS.René On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: We have a tissue sample that was processed and paraffin embedded.  We URGENTLY

Re: [Histonet] DIF on paraffin embedded tissue

Interesting comments. The use of this sample depends on if the antigen survives fixation and paraffin embedding. About 45-55% of our work is done with immunofluorescence on FFPE slides. we quench the autofluorescence with a CuSO4 treatment. James Watson HT  ASCP GNF  Genomics Institute of

Re: [Histonet] Are gloves required when cutting FFPE blocks?

You should have licked the block in front of them like one supervisor did when questioned about wearing gloves while sectioning! From: "goodwin, diana via Histonet" To: 'Rene J Buesa' ; "'histonet@lists.utsouthwestern.edu'"

Re: [Histonet] DIF on paraffin embedded tissue

I'm not sure that is the case in the grand scheme of things, it will depend upon the target that you need to stain via immunofluorescence. Technically we perform IF staining on frozen sections primarily because the antigen does not survive formalin fixation. Many people utilize IF techniques

Re: [Histonet] Are gloves required when cutting FFPE blocks?

We were cited for this by CAP. I need a specific reference to rebut or comply. Nothing specific to routine paraffin microtomy in CAP checklists. dg From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Wednesday, November 25, 2015 10:04 AM To: goodwin, diana; 'histonet@lists.utsouthwestern.edu'

Re: [Histonet] DIF on paraffin embedded tissue

Useless sample Sorry Sent from my iPhone > On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet > wrote: > > No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is > USELESS.René > > >On Wednesday, November 25, 2015 10:55 AM,

Re: [Histonet] DIF on paraffin embedded tissue

I, agreeing with Liz, would suggest looking out a IF protocol for FFPE tissues - I've recently concluded a validation of PLA (proximity ligation assay) in: cells in culture, FFPE cell pellets and FFPE xenografts. The signal was consistently there (although a drop in expression - from fixative

Re: [Histonet] Are gloves required when cutting FFPE blocks?

Never been cited by cap or clia for not wearing gloves There are hundreds of papers supporting the face that formalin fixed tissues will not spread disease all ER docs cite them when you go in for cutting your finger and ask about transmission CJD cases are the exception Sent from my iPhone >

[Histonet] DIF on paraffin embedded tissue

We have a tissue sample that was processed and paraffin embedded. We URGENTLY need to recover the tissue and perform Immunofluorescence on the sample. Does anyone have a procedure. HELP madeathri...@pastnashville.com ___ Histonet

Re: [Histonet] DIF on paraffin embedded tissue

Also agree. Lots of people do it. Search PubMed, J. Histo Cyto. If you have the appropriate filters, using Cy dye secondary's can help move away from any autofluorescence. Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K

Re: [Histonet] Are gloves required when cutting FFPE blocks?

NOT AT ALL, unless you are sectioning a suspected or known Creutzfeldt-Jakob's disease case. Check CAP regulations.René On Wednesday, November 25, 2015 9:56 AM, "goodwin, diana via Histonet" wrote: Can anyone provide a reference as to whether or not

Re: [Histonet] DIF on paraffin embedded tissue

No way...no how Sorry! Sent from my iPad > On Nov 25, 2015, at 11:10 AM, Simmons, Christopher via Histonet > wrote: > > Useless sample > Sorry > > Sent from my iPhone > >> On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet >>

[Histonet] Toluidine blue stain fo plant tissue

Could anyone give me the concentration (? 0.1-0.05%) and the exact technique/protocol to use. I am staining shoot apical meristems not leaf or root. Regards Peter Noyce PhD student. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] DIF on paraffin embedded tissue

Obviously it depends on what you are looking at, but I have had much success with DIF on paraffin sections Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.org

[Histonet] Stains specific to nervous vs. endocrine tissue on insects

Dear Histonetters: Could I be directed to stains specific to nervous vs. endocrine tissue on insects? Gracias, sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free

Re: [Histonet] DIF on paraffin embedded tissue

Maryann, Whoa, bad day. I'm sorry for you and for the patient. As for whether the testing usually performed in frozen section by Direct ImmunoFluorescence can be successfully done on FFPE, Liz Chlipala is probably the closest to answering this. This was likely a kidney or skin biopsy. The

Re: [Histonet] DIF on paraffin embedded tissue

By all means share your protocols plz Sent from my iPhone > On Nov 25, 2015, at 12:44 PM, Houston, Ronald via Histonet > wrote: > > Obviously it depends on what you are looking at, but I have had much success > with DIF on paraffin sections > > > Ronnie

Re: [Histonet] DIF on paraffin embedded tissue

Teri Excellent point and to add to that most IF techniques that are employed on FFPE are not direct they are indirect and consist of an unlabeled primary and then a secondary that is conjugated with an alexa fluor or something similar at least that is how we approach IF on FFPE tissues here.

[Histonet] Are gloves required when cutting FFPE blocks?

Can anyone provide a reference as to whether or not gloves are required when cutting routine FFPE blocks? Diana Goodwin UMCPP Anatomic Pathology Supervisor Office 609-853-6808 Histology 609-853-16860 This e-mail transmission and any documents attached hereto contain information from