[Histonet] Validation Process or Procedure for the Shandon Cytospin 4 and the Sorvall ST 8 Centrifuge

2016-01-20 Thread ian bernard via Histonet
Does anyone have a validation protocol to share? Mr B ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histonet Digest, Vol 146, Issue 18 ( Microtome Thick and Thin issues)

2016-01-20 Thread Brent Adams via Histonet
Tyrone I am in agreement with Carlos except low operating temperature can effect the lubrication of the slide mechanism by gelling up and causing the slide mechanism to skip and jump. Lubrication of the internal mechanics of the Microtome are very necessary on a regular basis. If volumes use is

Re: [Histonet] microtome issues: thick and no sections (Tyrone Genade)

2016-01-20 Thread Tyrone Genade via Histonet
Hello, Thanks to all who responded to my question. The solution, it seems was very simple. Several people reported similar experiences and issues with the specimen clamp not fitting tightly and that I should clean the clamp or block. My blocks are clean and I thought the clamp was too... but

[Histonet] Pinkerton's adaptation of machiavello's stain for chlamydophila on tissue sections

2016-01-20 Thread Julio Benavides via Histonet
Hi there, I was wondering if any of you had used the Pinkerton's adaptation of machiavello's stain for staining chlamydophila on parafin tissue sections and whether it works or not. I would also be very grateful if anybody can advise in any other technique working on tissue sections. We