[Histonet] unsubscribe

Please unsubscribe my email address Thank you! Pauletta A. Robbins ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Suggestions for making glutaraldehyde penetrate in between little setae of insects for microscopy

A little alcohol will lower the surface tension of aqueous solutions. Then there's always DMSO. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Feb 26, 2016 at 8:15 AM, Jorge A. Santiago-Blay via Histonet < histonet@lists.utsouthwestern.edu>

Re: [Histonet] Suggestions for making glutaraldehyde penetrate in between little setae of insects for microscopy

Double PPE if you use DMSO Sent from my iPhone > On Feb 26, 2016, at 2:41 PM, Jay Lundgren via Histonet > wrote: > > A little alcohol will lower the surface tension of aqueous solutions. Then > there's always DMSO. > > > Sincerely, > >

Re: [Histonet] Image analysis software

Judi Through the years we have used a wide variety of software applications, we have been performing image analysis since 2006, we started whole slide scanning in 2007. We have used various modules from Zeiss, Image Pro, Aperio's Toolbox, Definiens and free shareware such at Image J. There

[Histonet] h. pylori

Hi histonetters, We filter the H.pylori antibody with the filters that come with the Ventana Prep kits. Elaine Dooley Shands Teaching Hospital 352-265-0111 ext 72117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] H. pylori by IHC

Recently someone placed a query regarding the precipitate with Cell Marques H. pylori marker. The response was to filter it before use to clean it up. May I ask how it is being filtered? We have the same problem and it makes the slide difficult to interpret. Joanne Clark, HT BAAS Director

[Histonet] Friday Fun (non-work related blog post, easy to ignore)

Anyone moving to Chicago area? http://www.chicagonow.com/downsize-maybe/2016/02/to-the-family-who-will-buy-our-house-whoever-you-are/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203

Re: [Histonet] CAP

Bernice We are not a clinical lab, we are a GLP compliant lab and we have a procedure that addresses this and everything else about reagent preparation. I will put the procedure and forms that we use on the NSH BLOCK for anyone who is interested. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

[Histonet] glycine method to remove aldehyde induced autofluorescence

Dear All, Some person kindly mentioned my name as a source for the glycine method to remove aldehyde induced autofluorescence. We liked the simplicity of this method, plus gentle to tissue sections. This was the original information but we modified it. I have seen concentrations of

Re: [Histonet] auto-fluorescence

Dear Joost, Yes and to read up on this, go to this website for an excellent, well referenced free pdf i.e. Autofluorescence, causes and cures. https://www.google.com/?gws_rd=ssl#q=autofluorescence+causes+and+cures+wrigh t+cell+imaging+facility They also posted a pdf on mounting medias for

[Histonet] HISTOPALOOZA III Georgia Society for Histology

Histopalooza! III welcomes Ely Klar, MS,HTL(ASCP) back as a presenter. Ely is an instructor at Columbus State University in Columbus, Georgia, home of the Cougars. (Cougars being the school mascot, not where all single women over 40 live.) Her workshop on microscopic anatomy is always well

[Histonet] Suggestions for alternative fluorescent nuclei stains (that are not DAPI) ?

Hello all, I perform immunohistochemistry (IHC) and immunofluorescence (IF) on fixed free-floating rat brain sections (~50um thickness). Currently I use Prolong Gold with DAPI as a mounting media as well as a stain for the nuclei. I am seeking an alternative (fluorescent) nuclei stain for

[Histonet] Suggestions for making glutaraldehyde penetrate in between little setae of insects for microscopy

Hello Histonetetrs: I am trying to fix insect wings for TEM. Less than ideal fixation appears to be caused by abundant setae on the surface of the wings. Those setae, trap air, thus making penetration of the fixative more difficult difficult. Interestingly, also the wings tend to curl upon

[Histonet] CAP

ANP21382. What kind of policy or SOP do you all have for this question? T asks how reagents are given an expiration. This includes but is not restricted to reagents where manufacturer does not specify a date. We date made up reagents as a 6 month expiration unless we know it doesn't last that

[Histonet] Suggestions for staining bacteria in FFPE decaled growth plate

Happy Friday, Histonetters! I have a puzzle that I could really use your expert knowledge on this morning. One of our pathologists has a decalcified FFPE mouse bone embedded longitudinally and has found some bacteria located in the growth plate. The bacteria has been confirmed with fluorescence,

[Histonet] Direct Hire for Histotech Job in Houston, TX

Happy Friday Everyone! I have a position in Houston, TX for Full Time/Permanent. The shift is Evening Shift. Please contact me for job description. Have a great day! Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572

Re: [Histonet] Image analysis software

Hi Jodi, I was using imageAnalysis from Olympus some years ago, bus since I discovered ImageJ there is no way I will go back to commercial stuff. Pros of ImageJ, apart from the obvious one that it is free, is that a lot of people is using it, so plenty of tutorials, tricks, how-to in

Re: [Histonet] Human nuclear antibody MAB1281

I had/have the same problem with that ab on FFPWS +/- AR Also negative, under same conditions: Serotec's 6970-1257, Abcam's ab5675 I use 2 abs on hu teratomas in mouse host: Takara clontech STEM121 antibody Abcam anti MTCO2 ab110258 Images to be seen on Histonet Images site Carl