Re: [Histonet] Cryostat Chirp

2016-05-03 Thread Elizabeth Chlipala via Histonet
Gary I think that might have to do with the fan motor inside the unit, it might need to be replaced. We had ours replaced a few years ago. Do you service/PM the cryostat yearly? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-394

[Histonet] Cryostat Chirp

2016-05-03 Thread Martin, Gary via Histonet
My Leica CM 1850 cryostat is making a chirping sound and I can't figure out what is doing this. Has anyone experienced this sound, if what was the resolve. Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwe

Re: [Histonet] PAS/Decal Question

2016-05-03 Thread Rene J Buesa via Histonet
My impression is that your problem is during the decalcification step. It cannot be hurried and has to be in EDTA at pH 7All reagents have to be prepared in pH7 phosphate buffer.The inconsistency resides in the fact that not all core Bx are the same regarding thickness, tissue condition or size.

Re: [Histonet] Histonet Digest, Vol 150, Issue 2

2016-05-03 Thread T H via Histonet
Good to know that I am not the only on that think Rene is negative to others. Hey Rene it's not just one person that thinks your a dirt bag. From: histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, May 3, 2016 12:00 PM To: histonet@lists.utsouthwe

[Histonet] Golgi Kopsch

2016-05-03 Thread Mca Werdler via Histonet
Hello Everyone, I am completely new to the technique, i tried several different protocols but it doesn't seemed to succeed that very well. Does anyone have a protocol for the Golgi Kopsch, where i have to include the tissue in the end with paraffin? Thank you all for your time Mwerdler UNAM Mex

[Histonet] No More Blog Posts -- Over and Out!

2016-05-03 Thread Blake Taylor via Histonet
I have been completely disappointed at the remarks and ugliness that I have seen lately on this post. I will be extremely unlikely to continue to use this list serve anymore due to the complete lack of professionalism and compassion that has been displayed of late. This blog post talk has certa

[Histonet] Job Opening in CA

2016-05-03 Thread Paula Keene Pierce via Histonet
| | | | | | Dear Friends & Family,I am seeking a Research Scientist I, Stem Cell Biology  for a stem cell therapy project that we just started. The funding has been  secured for the entire project. The position is available immediately and the description is shown below. If you know somebo

[Histonet] PAS/Decal Question

2016-05-03 Thread Marcum, Pamela A via Histonet
We are still having issues with our PAS stain on decaled bone marrows. The Pathologists in HemePath are seeing what they refer to as smudginess in cells on some areas of the completed PAS slides. We have looked at everything and cannot find where the issue is coming from at this point. We hav

[Histonet] HHV-8 RTU

2016-05-03 Thread Delray Beach Pathology Kari Simeone via Histonet
My vendor (Cell Marque/Sigma Aldrich) has a YEAR backorder on this IHC RTU. Does anyone have a vendor they purchase from that I can research? Thanks so much. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor The information contained

Re: [Histonet] Fwd:

2016-05-03 Thread Rene J Buesa via Histonet
As I see it, the best solution is "1"Even more: if the piece of tissue is large enough,→cut 1 section and stain → select at least 2 (+) areas→ divide the block into 2 blocks each containing one of those 2 areas and by doing so you would have duplicated the number of possible (+) sections.René