[Histonet] Pap stain without xylene

2016-05-25 Thread Mike Toole via Histonet
Thank you Beth, It’s good to know that someone else had tried this and had very good results. The method you suggest is very much in line with the recommendations from René. He did recommend using a drying oven at 60°C to help ensure complete removal of any water or alcohol. And, that absolute

[Histonet] Stamp staining in paraffin and smear samples

2016-05-25 Thread Julio Benavides via Histonet
Hi There, Is anyone using this technique to stain chlamydia in placental samples (working with sheep here)? I was looking for a working technique but it looks like it is not easy to find. Thanks for your thoughts! Julio Rene J Buesa via Histonet

Re: [Histonet] GHS vs. NFPA labeling

2016-05-25 Thread Cates, Julia via Histonet
Oops! I do not believe it is permitted:) I need to read more carefully before I send out emails!! See the link below for a recent blog entry addressing this very question. I do not believe placing a new label on an original container is not permitted. I would transfer to a secondary

Re: [Histonet] Pap stain without xylene

2016-05-25 Thread Rene J Buesa via Histonet
Hi Mike:The steps you desrcibe are wrong.After you finish staining your PAP smear, just wash them in the last ethanol → oven dry at 60ºC for 5 minutes or as required if the smear is too thick and when completely dried → coverslip.This final drying has to take place at temperatures above room