Re: [Histonet] Modified Movat's Pentachrome stain

2016-09-06 Thread HirataMasahiro
Angela, I replace saffron with Gardenia Fruits Extract (Tokyo Chemical Industry, TCI America in US, C1527) for Movat's and HES. It has no C.I. number, but contains crocin dye (CAS 42553-65-1) ​extract the dye with ethanol (5-6g/100ml) for 2-3days and filtrate. I hope this will help ​ for you

[Histonet] New Bone vs. Old Bone Stain

2016-09-06 Thread Jessica Riggleman via Histonet
Hello, Can anyone recommend a stain that differentiates new (woven) bone vs. old (lamellar) bone? We are looking for bone growth, and biomaterial was used to facilitate. We are using a rabbit femoral defect and a rabbit PLF model. Protocols/Input for MMA thin/thick sections and formalin fixed