Hi Allan,
The flakes are alum precipitates.
I would suggest filtering the haematoxylin before use.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western
Hello all,
Does someone know what would cause dirty flakes of light purple that showed
across the whole slide?
http://i.imgur.com/1nIXwAy.jpg
Reagents are relatively fresh but the H stainer hadn't been used for a
week over the holiday break.
Is it surface film on the hematoxylin? I didn't
Completely agree...it sounds like the tissue got freeze dried and I doubt
if any staining you might get after lots of work will be completely
reliable.
Loralei
On Jan 3, 2017 11:02 AM, "Caroline Miller via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:
> I hate to say it but I think
I hate to say it but I think these tissues are toast. It seems they had bad
fixation or handling to start with, and I would question whether the
tissues were left out to dry before fixation or freezing. Or whether they
got fixed and then sucrose infiltrated (which is my method of choice if the
I got the following from a grad student here at Penn State. I am not sure how
to solve his problem if possible. Does anyone have any suggestions I can
forward?
Roberta Horner
Animal Diagnostic Lab
Penn State University
"I am having some difficulties sectioning mouse tumor samples for
For those of you working in smaller hospital Histology labs (not staffed 24/7)
- How does your OR/Lab handle getting specimens into fixative?
We currently use small/med prefilled containers for smaller specimens. Does
the OR dispense formalin into larger containers? Do you refrigerate unfixed
