[Histonet] Section adherence issues (IHC)

Hi Greg, Permit me a few thoughts on factors not mentioned in your email: * Do your slides 'look' baked/is the wax melted when they are removed from the oven? * Also, is there variance in your oven temperature? * Especially vexing is the fact that control sections are lifting. Are

Re: [Histonet] Histonet Digest, Vol 164, Issue 9 slide adhesion

Greg, It sounds like 2 or 3 problems and LEEP specimens are partially heat fixed, giving addition artifacts related to poor adhesion. 1. Short processing cycles w insufficient paraffin times or dirty xylenes can do this. Double your maintenance or try the radical by removing formalin from

Re: [Histonet] IHC sections adherence

Hi Greg, I use BONDS and Apex slides as well. What we do with tissue that washes (usually heavy tumor and some harsher stains like CD34) is leave the tissue unbaked at room temp for 12 hours and then bake at 60 degrees for 30 minutes. I don't know if you are including your control tissue on the