Re: [Histonet] Elastic Stain

In the UK clinical lab I started in we used Miller's elastin, which I think was a resourcinol formulation (digging from 20 years ago in my brain). I tried to find that in the US but never did. I loved that stain, worked well every time! We would counterstain with a van-Giesen, looked stunning! the

Re: [Histonet] Metal embedding molds-large

hey, we do all our embedding in those molds, and here is what I suggest: 1 - Make sure to have enough wax in the back of the molds, all the way until it is on the lip of the cassette - you may need to refill a bunch of times because the wax drains out (why regular sakura cassettes do not fit in

Re: [Histonet] Metal embedding molds-large

Sorry! That should be TSP - trisodium phosphate - not TCP, which might make it worse. Bryan Bryan Llewellyn wrote: This used to be a common problem years ago. It is due to crud buildup on the metal. Boil them with TCP for half an hour, then thoroughly wash them in cold water. Coat them

Re: [Histonet] Metal embedding molds-large

This used to be a common problem years ago. It is due to crud buildup on the metal. Boil them with TCP for half an hour, then thoroughly wash them in cold water. Coat them with a VERY light smear of glycerol before you use them, preferably each time. That should help. Bryan Llewellyn. Diane

Re: [Histonet] Metal embedding molds-large

mold release Virus-free. www.avg.com <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> On Wed, Nov 8,

[Histonet] Metal embedding molds-large

We are using large metal molds to embed mouse brains. We are having a hard time getting to block out of the molds, the paraffin blocks are sticking. Sometimes they are coming out cracked. Sometimes the cassette comes off the paraffin block. Any idea why this is happening? Any advice on how

[Histonet] NK Cell markers for Mouse in Paraffin

Hi all - anyone out there have an antibody that they like for NK Cells in a mouse model - I am trying to work with a mouse monoclonal (MOM) with polymer technology. Looking for assistance from labs that have established protocols. We have tried enzymatic and heat retrieval ..not getting good

[Histonet] mounting medium

Hello to all Can anyone suggest which mouting medium is best that will help preservation of staining properties without creating fading of stains and bubbles on long run. Thanks alot ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Division of Path cases

We give the worklist to the chief Pathologist and let him make the assignments on a daily basis. They are usually divided up by complexity. In the absence of the Chief Path, then I make the assignments. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital

Re: [Histonet] Elastic Stain

We love the Aldehyde Fuchsin with a fast-green counterstain. Once the initial working stain is made up, it is a fast and easy stain to perform, not to mention just pretty - purple fibers against a green background. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer