Re: [Histonet] Acid Phosphatase Protocol

2018-01-25 Thread Tony Henwood (SCHN) via Histonet
Hi April, Try this Control tissues: liver, kidney and prostate Fixation and Sectioning: Air dried unfixed 8µm frozen sections Solutions: 1. Substrate solution Naphtol AS-B1 phosphate 0.005g (Fluka 70494 1g) Dimethylfomamide0.5ml Warning: Suspected Carcinogen

Re: [Histonet] Image analysis on breast markers

2018-01-25 Thread Liz Chlipala via Histonet
Teresa CAP should be releasing a paper soon, within the next couple months. Quantitative Image Analysis (QIA) for HER2 Immunohistochemistry for Breast Cancer, it will address the question that you have. Keep any eye out for it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory

[Histonet] Image analysis on breast markers

2018-01-25 Thread Lima, Teresa via Histonet
Hello all, I am wondering if any of you out there that do image analysis of breast markers have a technologist select the field of view to be analyzed by the system. Our techs do this with no supervision by a pathologist. In many instances the pathologist changes the analyzed result without logg

Re: [Histonet] Histotechnician open positions

2018-01-25 Thread Willis, Donna G. via Histonet
Good Afternoon Histology world, Baylor Scott and White, Baylor University Medical Center Dallas has 2 open position on the evening/night shift, in the routine Histology laboratory. 1 position is full time from 6pm to 2:30am and the other is a part time position from 2am to 6am. Application to

[Histonet] Leica Reichert-Jung cryocut 1800 cryostat manual

2018-01-25 Thread Alex Horkowitz via Histonet
Hello All, I am in need of a manual for a Leica Reichert-Jung cryocut 1800 cryostat with 2020 microtome. Any tips for sectioning mouse lung with this system would be much appreciated as well. Cheers, Alex Horkowitz San Diego State University 2nd Year Cell & Molecular Biology MS Student alexho

[Histonet] Acid Phosphatase Protocol

2018-01-25 Thread Oler, April via Histonet
Good afternoon, I am writing to you all because I am in search of Acid Phosphatase muscle protocols that do not utilize sodium barbital. I'm hoping to eliminating sodium barbital from my lab in the near future. If anyone is willing to share their protocol, or advice, I would very much appreciat

[Histonet] tracking slide filing

2018-01-25 Thread Minikus, Carrie A. via Histonet
We would like to get an automated tracking system sometime in the future; however, right now it is not in our budget. We currently have no tracking system for who is filing our slides away into the file drawers and we have been having some real issues with slides not being able to be found due t

[Histonet] Making Bulk Reagents for Ventana

2018-01-25 Thread Nutter, James E via Histonet
Hi Everyone, Has anyone or does anyone make the bulk reagents for the Ventana Ultras? (LCS, SSC, Reaction Buffer, CC1, CC2) If so: How to turn off the tracking system for bulk reagents? Formulations? Success? Thank you all, James Nutter Chantilly, VA _

[Histonet] Used equipment

2018-01-25 Thread Campbell, Tasha M. via Histonet
Anyone know who to contact to sell used equipment to? Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu h