Hello Histonet,
I am just starting a project using LCM for RNA extraction. So far, our
tissues haven't stayed on the slides very well. We are using the Acrturus
LCM staining kit.
We've taken meniscus at 10, 20, 30, and 40uM sections and started the
staining process with (10mins at room temp) and
This year our meeting will be held at Callaway Gardens Lodge, Pine Mountain,
Georgia. Remember we offer free membership. The all-inclusive registration
fee is $135, which is a steal these days for CEUs. Jack Ratliff will be back
this year as a speaker. Woohoo, can't wait to catch up with him,
I agree Terry,
The TMA slide is a very economical and powerful way to validate with
minimal slides needed.
Colleen Forster
On Tue, Mar 20, 2018 at 12:54 PM, Terri Braud via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Just another note: You can order unstained tissue microarrays with
Just another note: You can order unstained tissue microarrays with the
prerequisite number of cases, both positive and negative, and stain your
validation all on one slide. I've done this for years and for 3 different
validations of entire IHC platform changes, ranging from 40 to over 100
ant
Cap states, "should test a minimum of 10 positive and 10 negative tissues".
Does not state "cases" but "tissues", so we used a block for positive and
negative from the same case.
Thanks,
Tim
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