Hi,
I got some cadaver tissue floating in "LTP embalming fluid w/ phenol".
Can I put those straight into the processor? Or has to be rinsed/soaked before?
If so what should I use?
My Internet searches did not bring up anything useful.
Thank you!
Hanna Preiszner
ETSU/QCOM Dept of Pathology
I have never (3 different places, 30+ years) heard of requiring special stains
to be performed under a hood. Certainly, there are a few reagents that might
need benefit from a hood (concentrated Ammonium Hydroxide) but generally, they
are performed on an open counter next to a sink. If you
Help? we have a student with one of the online schools and we're doing
specials like it is 1972. She has to do a Al Blue 2.5 w Hyaluronidase
digestion. We haven't done this since the development of clinical IHC two
dozen years ago -
Is there any other way to acheive this outcome on a colon
25+ years in Histology, half the places I worked had vents on the wall near the
countertop where we did specials, otherwise it was on the counterclosest to the
sink. The only times we were time doing anything under a hood was for frozen
section staining because of the fresh tissue, mixing
I guess the question is, who told you this and what is their reference?
Most special stains don't require a hood. Only those that have volatile
chemicals or odors that may be offensive (ie, ammonium sulfide). We have some
areas we do specials without a hood. Our main special stain areas have a
That is certainly an odd request…
I have never performed any special stains under a hood
any place I have ever worked.
Always done on a counter top near a sink…
Is someone in your department extremely sensitive to odors? Or have asthma?
We have a tech who experiences attacks if there are certain
Fellow Histonet Members -
We are in the process of moving our IHC bench from the Leica BOND to the ROCHE
ULTRA and are in the mist of antibody validations.
We are having great difficulty in finding negatives to validate for MSH2 and
MSH6.
Would anyone have any loss of expression MSH2 and MSH6
