Hi All,
I have a validation question - we are looking into buying an RUO rated IHC
stainer for a future multiplexing project. Roche's Discovery Ultra to be
specific.
I have a pathologist that is convinced that we can run our clinical
antibodies on it as an LDT and still bill for the work. The
You are right!
Thank you for correcting me.
Surely sialomucins will be positive for PAS and H.acid will be negative?
Curious-illy
Carl
Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL
020 7848 6813
The hyaluronidase is digesting out hyaluronic acid, a component found in
connective tissue mucins. This reaction is used to differentiate connective
tissue mucins from epithelial mucins. Hyaluronic acid and epithelial
sialomucins will give the same staining results, both positive for alcian
Rinse in dist water ( or tap water) x3 over 30 mins
No big deal to do this but nice to get rid of extra smells in your processing
reagents ( depending on where you start processing: I start in 90%
alcoholsure, I ask for recd specimens to be rinsed in water then
transferred to 70% alcoholÂ
Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab
pH1 ( highly sulphated mucins only) on a sequential slide?
Sure, instead use Alcian blue CEC method?
I will be interested to read other responses to your request.
Mucin-illy
carl
Carl Hobbs FIBMS
Histology and
Here is the approximate chemical make-up of LTP with Phenol. (LTP stands for
Long Term Preservation)
Ethanol 48.%
Formaldehyde 20.%
Ethylene Glycol 4.%
Phenol 11.%
Glutaraldehyde 4.%
Due to the presence of Ethylene Glycol, I would let the tissue set in a
separate container of 10% Neutral
Afternoon,
Looking to see if anyone has some tipsheets on how to pull embedding logs or
accession logs from winsurg. I am by no means familiar with the system so if
you could send me step by step instructions I would really appreciate it!!
Thanks!
Jessica Vacca
HCA Epic Anatomic Pathology Lead
