Good Afternoon Listers,
Thanks to all who answered my question about retention of unstained
slides. I had several people weigh in on the topic and we have created an
unstained slide policy based on their advice and the literature that was
kindly sent to me.
The consensus is antigenicity
Please ask why Osmium is required?
Good advice given so far.
If it is to "stain" for myelin, surely an anti Myelin antibody should be used?
Visualise using a complementary Fluorophore to that which is indicated but not
explained, in your request?
Good luck
Carl Hobbs FIBMS
Histology
Hello Histonetters,
It is hard to believe the summer is almost over. The kids are going back to
school and Labor Day is just a few days away!
In a few more weeks -Halloween; Then Thanksgiving, Christmas and New Year's
Day!!
Looking for a new job in the Fall?
Planning a job change after
I would suspect osmium would destroy the fluorescent proteins the PI wants to
detect, while staining fat droplets nicely. You can always ask your friendly
EM lab to do the staining to find out, they have to use it and are familiar
with the proper ways to deal with it.
Osmium Tetroxide is highly toxic. Storage is an issue and working with it, you
must be under a hood with eye protection. I think most (if not all) labs have
banned this substance? If you do not “have” to work with this material, I would
recommend avoiding it. Just my opinion, thanks…
Shannon
Hi all you fellow Histonetters!
My query of the day to you is: can you recycle xylene that has been
contaminated with formalin? (yes, you heard me correctly, formalin)
Thanks for any insight!
L
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Histotechs:
I am looking a protocol to stain osmium tetroxideIs anybody can share their
thoughts...on a frozen section.
I attached an idea of how this person wants me to follow his protocol with this
staining but I kind of lost...
my PI request:
I'll be perfusing the mice with saline
