Re: [Histonet] AFB STAIN

2018-10-03 Thread Tony Henwood (SCHN) via Histonet
We do, It works well Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the

[Histonet] Annual Competency

2018-10-03 Thread ADESUPO ADESUYI via Histonet
Hi, I am in the process of updating the competencies for histotechs and lab Assistant in our lab. I will appreciate it, if you guys could share. Thanks, Ade ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] AFB STAIN

2018-10-03 Thread ADESUPO ADESUYI via Histonet
Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Oil Red O

2018-10-03 Thread Amos Brooks via Histonet
Hi, I have seen this too. I mitigate the problem by making it up in 50 ml increments and staining them flat. I draw from around the middle of the Falcon tube I make it up in. It tends to precipitate so you could filter it. Don't bother re-using the reagent. It's cheap & easy to make up. I

Re: [Histonet] Oil Red O

2018-10-03 Thread Caroline Miller via Histonet
Hi Reuel, In my experience this is the ORO crystallizing out on the section. I would first filter your staining solution, then keep your staining times to as short as you can. Also changing between 37 and room temperatures may be useful too. mills ᐧ On Wed, Oct 3, 2018 at 8:39 AM Reuel Cornelia

[Histonet] Oil Red O

2018-10-03 Thread Reuel Cornelia via Histonet
I was wondering if you could help me know why our Oil Red O have some black snowflakes on our fat tissue hours after staining or after 24 hours more snowlflakes precipitation occurs. The staining was reference was from Lillie RD, Ashburn. Please note that our staining works well between two to

[Histonet] slide folders with space for blocks too

2018-10-03 Thread Caitlin Hesketh via Histonet
I want to order some folders that have space for blocks to go with the slides. I swear this exists - I remember very clearly bringing it up as a suggestion at my last job and the pathologist there shutting it down instantly (he didn't want to be responsible for checking the block against the slide