Gudrun-
Assuming this is a new issue with a recent reagent change, I suspect that the
alcohol used for the clean cycle was not 100% (maybe 95%?).
The ability of your alcohol to hold the paraffin in solution is lost as the
percentage of water increases.
I also expect your solvent was mostly
Either: 1) the ShellSol was over-used and could no longer clean/dissolve the
paraffin so the remaining paraffin ended up in the alcohol 2) There was water
in the cleaning alcohol (we always use 100% alcohol - do you always use 96%?
3) the cleaning ShellSol and cleaning alcohols were switched
Hi Histonetters,
How are you doing today? This e-mail is a little different than the normal
e-mail that I send out because I am working on a project and I need your
help I do have some great jobs but first
I am doing some research on how to get the word out on histology as a
profession. I am
Hi Everyone,
We are a dermatology center here in norcal and ocassionally our physicians are
on call for stat cases at local hospital. Can our physician bring the biopsy
from that stat case so we can do the technical component too so we can then
bill both the professional and technical?
Hi Greg,
It has been a problem for months across hundreds of kits. We added some washes
and more dips in the DI water after the run to the protocol. It helps a little
bit. I am going to try a more things in the next few weeks and I will keep the
community updated.
-Original Message-
Dear all!
I have a question for those, who are familiar with the VIPs from Sakura.
Last time we changed the reagenses the cleaning-ethanol (96%) was very milky
and even was full of many small particles. It was a paraffin-soup.
What is the cause for such a case? We have been using the organic
Hi Linda,
Is this a problem that has just started or has the red kit always had this
artefact?
- If it just started, perhaps it is a problem with a particular kit lot
number (or numbers). If so, get Leica Tech service involved to see if
anyone else has reported a problem.
- If it
