Re: [Histonet] guinea pig IHC

2019-01-29 Thread Colleen Forster via Histonet
following. I haven't done frozen stains on GP only FFPE...interested in what others have to say. Colleen On Tue, Jan 29, 2019 at 1:58 PM Jan Shivers via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Has anyone ever performed IHC on frozen sections of guinea pig tissue? I > am experien

[Histonet] guinea pig IHC

2019-01-29 Thread Jan Shivers via Histonet
Has anyone ever performed IHC on frozen sections of guinea pig tissue? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising

Re: [Histonet] Cameras in the gross room

2019-01-29 Thread Miranda Giorgi via Histonet
Hello Izak, The cameras at embedding show a view of the embedding hot plate and embedders lap. I would be surprised to meet a histo tech who has never experienced a piece of tissue popping out of a cassette when opening it up. The cameras are useful for identifying where the tissue might have

[Histonet] Can I Warm You Up With some Sizzlin' Hot Histo Opportunties?

2019-01-29 Thread Pam Barker via Histonet
Hello Histonetters, I hope you are having a great week. I wanted to send a quick note to tell you about the positions that I am working on and am most excited about.  Why am I excited about these positions? Because each of these clients was asked if I had someone for you today would you be ready

Re: [Histonet] glass slides

2019-01-29 Thread Dessoye, Michael via Histonet
That's interesting, we've been seeing warping with the Leica coverslips. I wonder if there's something in common in their manufacturing. We've been using a mixture of Superfrost Plus (Fisher), Basix (Fisher), and Platinum line (Mercedes Medical) all with good results. The 'Basix' seems to be

[Histonet] Cameras in the gross room

2019-01-29 Thread Izak Dimenstein via Histonet
Dear Miranda Giorgi: What is the purpose to have cameras, especially at the embedding station? What kind of cameras do you use? Thank you, Izak Dimenstein ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mail