Reguarding:
Hello fellow Histonetters
I would like to ask you a question about IHC staining and derm cases. I am
seeing a peculiar issue going on, where the melanocytes in the middle of the
tissues are staining pretty well but when you get to the ends of the tissues
either shaves or ellipses,
Doesn't sound like a fixation issue to me. Could the tissue be drying out
before it's placed in formalin? Also, are these specimens inked for assessment
of margins? I've seen ink interfere with immunoreactivity.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman,
If the tumor is ER/PR negative the first thing I do is to look for an internal
positive control (immunoreactive benign breast epithelium). In my experience,
the majority of these cases have internal positive controls to validate the
negative ER/PR results. When I don't see internal positive
Hello fellow Histonetters
I would like to ask you a question about IHC staining and derm cases. I am
seeing a peculiar issue going on, where the melanocytes in the middle of the
tissues are staining pretty well but when you get to the ends of the tissues
either shaves or ellipses, they are not
Hi Karen,
As mentioned by others "decay" is not likely going to be an issue. More
concerning for you could be not knowing how those tissues were handled
prior to processing 10 years ago.
Presumably, you now track cold ischemic times and have standardized your
fixation protocols for breast tissues
