My preference, whenever possible, is to steer people towards using normal
tissues for controls in IHC. They are consistent in quality and appearance and
fixation and processing can be better controlled.
I suggest you use Appendix for Ki67 and evaluate the lymphoid follicles plus
there are some
We had several cases last Friday that showed "optically clear nuclei"
(primarily lymphocytes) on H Have you seen this artifact and, if so, what
is it due do? I will be happy to e-mail a photo to anyone interested. Thank
you as always for your help.
Richard
Richard W. Cartun, MS, PhD
Hello Histonet!
Looking for some advice regarding ER/PR/Her2 controls. I'm able to buy
commercially TMA blocks containing varying levels of positivity for ER, PR, and
Her2. This work nicely because you can have a negative and varying levels of
positive control as a same-slide control. But
We are looking for IVD antibodies for C1q and C4d for use on frozen tissues
(renal). What vendors are folks using for these direct immunofluorescence
stains?
Thanks in advance for your help.
Martha Ward
Wake Forest Baptist Health
Winston-Salem, NC 27157
